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Literature summary for 4.1.1.23 extracted from
- Determination of the mechanism of orotidine 5'-monophosphate decarboxylase by isotope effects (2000), Biochemistry, 39, 4569-4574.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K93C | inactive mutant, rescue of the mutant with bromethylamine restores activity | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | Saccharomyces cerevisiae | catalyzes the critical final step in the pyrimidine biosynthetic pathway | UMP + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| orotidine 5'-phosphate = UMP + CO2 | mechanism | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | mechanism, carbanion intermediate is stabilized by simple electrostatic interaction with Lys-93, the driving force of reaction is ground state destabilization resulting from charge repulsion between the carboxyl of the substrate and Asp-91 | Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
| Orotidine 5'-phosphate | catalyzes the critical final step in the pyrimidine biosynthetic pathway | Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | very high catalytic proficiency | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor requirement | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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