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Literature summary for 4.1.1.20 extracted from

  • Marjanovic, A.; Ramirez-Palacios, C.J.; Masman, M.F.; Drenth, J.; Otzen, M.; Marrink, S.J.; Janssen, D.B.
    Thermostable D-amino acid decarboxylases derived from Thermotoga maritima diaminopimelate decarboxylase (2021), Protein Eng. Des. Sel., 34, gzab016.
    View publication on PubMed
Search for more literature for 4.1.1.20

Protein Variants

Protein Variants Comment Organism
E315T mutant has lost most of the activity toward 2,6-diamonopimelate and has the highest activity toward 2-aminopimelate observed Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 meso-2,6-diaminoheptanedioate wild-type, pH 7.8, 30°C Thermotoga maritima
0.32
-
 2-aminopimelate mutant E315T, pH 7.8, 30°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1K5
-
-
Thermotoga maritima DSM 3109 Q9X1K5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.1
-
 substrate 2-aminopimelate, mutant E315P, pH 7.8, 30°C Thermotoga maritima
14.7
-
 substrate 2-aminopimelate, wild-type, pH 7.8, 30°C Thermotoga maritima
21
-
 substrate 2-aminopimelate, mutant E315F, pH 7.8, 30°C Thermotoga maritima
22.6
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315P, pH 7.8, 30°C Thermotoga maritima
26.3
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315T, pH 7.8, 30°C Thermotoga maritima
26.6
-
 substrate L-ornithine, wild-type, pH 7.8, 30°C Thermotoga maritima
32.3
-
 substrate alpha-aminoadipate, mutant E315N, pH 7.8, 30°C Thermotoga maritima
32.7
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315L, pH 7.8, 30°C Thermotoga maritima
36
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315F, pH 7.8, 30°C Thermotoga maritima
36.5
-
 substrate 2-aminopimelate, mutant E315L, pH 7.8, 30°C Thermotoga maritima
41
-
 substrate 2-aminopimelate, mutant E315Q, pH 7.8, 30°C Thermotoga maritima
42
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315N, pH 7.8, 30°C Thermotoga maritima
62
-
 substrate 2-aminopimelate, mutant E315M, pH 7.8, 30°C Thermotoga maritima
82
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315Q, pH 7.8, 30°C Thermotoga maritima
111
-
 substrate meso-2,6-diaminoheptanedioate, mutant E315M, pH 7.8, 30°C Thermotoga maritima
482
-
 substrate 2-aminopimelate, mutant E315T, pH 7.8, 30°C Thermotoga maritima
675
-
 substrate meso-2,6-diaminoheptanedioate, wild-type, pH 7.8, 30°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminopimelate substrate of mutant E315T, very poor substrate of wild-type Thermotoga maritima 6-aminocaproate + CO2
-
 ?
2-aminopimelate substrate of mutant E315T, very poor substrate of wild-type Thermotoga maritima DSM 3109 6-aminocaproate + CO2
-
 ?
alpha-aminoadipate substrate of mutant E315N Thermotoga maritima ? + CO2
-
 ?
alpha-aminoadipate substrate of mutant E315N Thermotoga maritima DSM 3109 ? + CO2
-
 ?
L-ornithine substrate of wild-type, no substrate of mutants in residue E315 Thermotoga maritima putrescine + CO2
-
 ?
L-ornithine substrate of wild-type, no substrate of mutants in residue E315 Thermotoga maritima DSM 3109 putrescine + CO2
-
 ?
meso-2,6-Diaminoheptanedioate
-
 Thermotoga maritima L-Lysine + CO2
-
 ?
meso-2,6-Diaminoheptanedioate
-
 Thermotoga maritima DSM 3109 L-Lysine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DAPDC
-
 Thermotoga maritima
TM_1517
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.075
-
 meso-2,6-diaminoheptanedioate wild-type, pH 7.8, 30°C Thermotoga maritima
0.2
-
 2-aminopimelate mutant E315T, pH 7.8, 30°C Thermotoga maritima