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Literature summary for 4.1.1.20 extracted from

  • Crowther, J.M.; Cross, P.J.; Oliver, M.R.; Leeman, M.M.; Bartl, A.J.; Weatherhead, A.W.; North, R.A.; Donovan, K.A.; Griffin, M.D.W.; Suzuki, H.; Hudson, A.O.; Kasanmascheff, M.; Dobson, R.C.J.
    Structure-function analyses of two plant meso-diaminopimelate decarboxylase isoforms reveal that active-site gating provides stereochemical control (2019), J. Biol. Chem., 294, 8505-8515.
    View publication on PubMed
Search for more literature for 4.1.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
structure to 1.9 A resolution Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
 meso-2,6-diaminoheptanedioate pH 8.0, 30°C Arabidopsis thaliana
5.1
-
 meso-2,6-diaminoheptanedioate pH 8.0, 30°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 and 100000, analytical ultracentrifugation Arabidopsis thaliana
100000
-
 and 50000, analytical ultracentrifugation Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q949X7 isoform DAPDC1
-
Arabidopsis thaliana Q94A94 isoform DAPDC2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,6-Diaminoheptanedioate
-
 Arabidopsis thaliana L-Lysine + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer and monmer, 2 * 53807, calculated from sequence. Dimer is predominant Arabidopsis thaliana
dimer and monmer, 2 * 54240, calculated from sequence. Dimer is predominant Arabidopsis thaliana
monomer and dimer, 1 * 53807, calculated from sequence. Dimer is predominant Arabidopsis thaliana
monomer and dimer, 1 * 54240, calculated from sequence. Dimer is predominant Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At3g14390 locus name Arabidopsis thaliana
At5g11880 locus name Arabidopsis thaliana
DAPDC1
-
 Arabidopsis thaliana
DAPDC2
-
 Arabidopsis thaliana
LYSA1
-
 Arabidopsis thaliana
LYSA2
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 meso-2,6-diaminoheptanedioate pH 8.0, 30°C Arabidopsis thaliana
0.014
-
 meso-2,6-diaminoheptanedioate pH 8.0, 30°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism binding of PLP initiates rearrangements within the active site, specifically the reorientation of Arg169, His218, and His22, which is propagated to the active-site loop, through Arg169. The substrate, meso-diaminopimelate, is positioned through interactions with conserved active-site residues to form an additional hydrogen bond to His220. This stabilizes the closed active-site loop conformation, mediated through Arg169, and can only occur when the D-stereocenter is oriented for decarboxylation Arabidopsis thaliana
physiological function expression of DAPDC1 rescues Escherichia coli that harbors a mutation in the gene encoding DAPDC. DAPDC1 is only able to rescue the mutant when its expression is repressed by glucose Arabidopsis thaliana
physiological function expression of DAPDC2 rescues Escherichia coli that harbors a mutation in the gene encoding DAPDC. DAPDC2 is only able to rescue the mutant when its expression is repressed by glucose Arabidopsis thaliana