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Literature summary for 4.1.1.20 extracted from

  • Gokulan, K.; Rupp, B.; Pavelka, M.S., Jr.; Jacobs, W.R., Jr.; Sacchettini, J.C.
    Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis (2003), J. Biol. Chem., 278, 18588-18596.
    View publication on PubMed
Search for more literature for 4.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor-diffusion in hanging drops at 18°C, crystals grow within 3-7 days in 0.004 ml hanging-drops containing 0.002 ml of DAPDC at 10 mg/ml and 5 mM lysine, equilibration against 0.5 ml well solution containing 24% polyethylene glycol mono-methylester 5000, 100 mM MES, pH 6.3, and 60 mM ammonium sulfate, crystals diffract to 2.6 A Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-2,6-Diaminoheptanedioate Mycobacterium tuberculosis
-
 L-Lysine + CO2
-
 ?
meso-2,6-Diaminoheptanedioate Mycobacterium tuberculosis H37Rv
-
 L-Lysine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIU7
-
-
Mycobacterium tuberculosis H37Rv P9WIU7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant DAPDC Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,6-Diaminoheptanedioate
-
 Mycobacterium tuberculosis L-Lysine + CO2
-
 ?
meso-2,6-Diaminoheptanedioate
-
 Mycobacterium tuberculosis H37Rv L-Lysine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DAPDC
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis