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Literature summary for 4.1.1.2 extracted from

  • Imaram, W.; Saylor, B.T.; Centonze, C.P.; Richards, N.G.; Angerhofer, A.
    EPR spin trapping of an oxalate-derived free radical in the oxalate decarboxylase reaction (2011), Free Radic. Biol. Med., 50, 1009-1015.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
S161A the mutant shows increased specific activity compared to the wild type enzyme Bacillus subtilis
S161T the mutant shows reduced specific activity compared to the wild type enzyme Bacillus subtilis
T165S the mutant shows reduced specific activity compared to the wild type enzyme Bacillus subtilis
T165V the mutant shows strongly reduced specific activity compared to the wild type enzyme Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the wild type enzyme contains 1.4 Mn2+ ions per monomer Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34714
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
mutant enzyme T165V, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
22
-
mutant enzyme T165S, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
29
-
mutant enzyme S161T, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
40
-
wild type enzyme, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
48
-
mutant enzyme S161A, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + H+
-
Bacillus subtilis formate + CO2
-
?

Synonyms

Synonyms Comment Organism
OXDC
-
Bacillus subtilis