Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
S161A | the mutant shows increased specific activity compared to the wild type enzyme | Bacillus subtilis |
S161T | the mutant shows reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
T165S | the mutant shows reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
T165V | the mutant shows strongly reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the wild type enzyme contains 1.4 Mn2+ ions per monomer | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34714 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
mutant enzyme T165V, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
22 | - |
mutant enzyme T165S, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
29 | - |
mutant enzyme S161T, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
40 | - |
wild type enzyme, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
48 | - |
mutant enzyme S161A, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | - |
Bacillus subtilis | formate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXDC | - |
Bacillus subtilis |