Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | induced by low pH, not by oxalate salts | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
recombinant OxdC, hanging drop vapour diffusion method, X-ray analysis | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
E162A | Mn2+-binding site 1 mutant, inactive | Bacillus subtilis |
E162Q | Mn2+-binding site 1 mutant, Vmax and kcat/Km is 1% that of wild-type | Bacillus subtilis |
E333A | Mn2+-binding site 2 mutant, 4-6% of wild-type activity at pH 4, lower Km for oxalate, kcat/Km is 25% that of wild-type | Bacillus subtilis |
E333Q | Mn2+-binding site 2 mutant, inactive | Bacillus subtilis |
R270A | Mn2+-binding site 2 mutant, kcat/Km is 3% that of wild-type | Bacillus subtilis |
R270K | Mn2+-binding site 2 mutant, kcat/Km is 43% that of wild-type | Bacillus subtilis |
R92A | Mn2+-binding site 1 mutant, inactive | Bacillus subtilis |
R92K | Mn2+-binding site 1 mutant, kcat/Km is 7% that of wild-type | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
oxalate | pH 4, 26°C, R270K mutant OxdC | Bacillus subtilis | |
2 | - |
oxalate | pH 4, 26°C, R92K mutant OxdC | Bacillus subtilis | |
4 | - |
oxalate | pH 4, 26°C, E333A mutant OxdC | Bacillus subtilis | |
8 | - |
oxalate | pH 4, 26°C, R270A mutant OxdC | Bacillus subtilis | |
14 | - |
oxalate | pH 4, 26°C, E162Q mutant OxdC | Bacillus subtilis | |
16.4 | - |
oxalate | pH 4, 26°C, wild-type OxdC | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus subtilis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | contains 2 Mn2+ per subunit, role in catalysis, mechanism, 2 Mn-binding sites, the N-terminal Mn-binding site 1 is catalytically active | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | Bacillus subtilis | involved in the elevation of cytoplasmic pH | formate + CO2 | - |
? | |
oxalate + H+ | Bacillus subtilis 168 | involved in the elevation of cytoplasmic pH | formate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34714 | - |
- |
Bacillus subtilis 168 | O34714 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant OXDC | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
oxalate = formate + CO2 | catalytic mechanism | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | catalytic mechanism involves the requirement of an active site proton donor: Glu-162, catalytic cycle, enzyme structure, N-terminal domain is the catalytically active domain, dioxygen-dependent reaction involves no net redox change | Bacillus subtilis | formate + CO2 | - |
? | |
oxalate + H+ | involved in the elevation of cytoplasmic pH | Bacillus subtilis | formate + CO2 | - |
? | |
oxalate + H+ | catalytic mechanism involves the requirement of an active site proton donor: Glu-162, catalytic cycle, enzyme structure, N-terminal domain is the catalytically active domain, dioxygen-dependent reaction involves no net redox change | Bacillus subtilis 168 | formate + CO2 | - |
? | |
oxalate + H+ | involved in the elevation of cytoplasmic pH | Bacillus subtilis 168 | formate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the cupin superfamily, bicupin | Bacillus subtilis |
OXDC | formerly YvrK | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
not active above | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
O2 | utilizes dioxygen as cofactor | Bacillus subtilis |