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Literature summary for 4.1.1.2 extracted from
- Oxalate decarboxylase from Collybia velutipes. Purification, characterization, and cDNA cloning (1991), J. Biol. Chem., 266, 23548-23553.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Flammulina velutipes |
General Stability
| General Stability | Organism |
|---|---|
| retains 45% of its activity after incubation with 10% SDS for 30 min at room temperature. Almost complete loss of activity after 30 min, in presence of 10% SDS | Flammulina velutipes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | competitive | Flammulina velutipes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
oxalate | - |
Flammulina velutipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE | Flammulina velutipes |
| 64000 | - |
x * 64000, glycosylated enzyme, SDS-PAGE | Flammulina velutipes |
| 560000 | - |
the high apparent MW obtained by gel filtration can be due to the tendency of certain glycoproteins to interact noncovalently in solution, gel filtration | Flammulina velutipes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxalate | Flammulina velutipes | induction by oxalate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flammulina velutipes | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 15% neutral sugar content | Flammulina velutipes |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Flammulina velutipes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 mg/ml protein, 0.02 M potassium acetate, more than 70% of the initial activity is retained after 4 months | Flammulina velutipes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxalate | - |
Flammulina velutipes | Formate + CO2 | - |
? | |
| Oxalate | induction by oxalate | Flammulina velutipes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE | Flammulina velutipes |
| ? | x * 64000, glycosylated enzyme, SDS-PAGE | Flammulina velutipes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
30 min, in presence of 10% SDS, retains 45% of its activity | Flammulina velutipes |
| 60 | - |
30 min, in presence of 10% SDS, complete inactivation | Flammulina velutipes |
| 80 | - |
20 min, 22% loss of activity | Flammulina velutipes |
| 96 | - |
10 min, complete inactivation | Flammulina velutipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Flammulina velutipes |
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