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Literature summary for 4.1.1.19 extracted from
- Expression and purification of recombinant arginine decarboxylase (SpeA) from Escherichia coli (2010), Mol. Biol. Rep., 37, 1823-1829.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in the T7 expression system as a cleavable poly-Histagged fusion construct in Escherichia coli strain Rosetta (DE3) pLysS | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 1 M lithium sulfate monohydrate, 0.1 M Na citrate tribasic dihydrate pH 5.6, and 0.5 M ammonium sulfate | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, HiTrap Q column chromatography, and DEAE Sepharose column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.151 | - |
crude extract, in 67 mM Tris-HCl (pH 7.5), 3.3 mM EDTA, 3.3 mM dithiothreitol, 0.04 mM pyridoxal 5'-phosphate | Escherichia coli |
| 2.8 | - |
purified enzyme, in 67 mM Tris-HCl (pH 7.5), 3.3 mM EDTA, 3.3 mM dithiothreitol, 0.04 mM pyridoxal 5'-phosphate | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine | - |
Escherichia coli | agmatine + CO2 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADC | - |
Escherichia coli |
| SpeA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
around 50°C | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.4 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | calculated from amino acid sequence | - |
5.1 |
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Release 2023.1 (January 2023)
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