Activating Compound | Comment | Organism | Structure |
---|---|---|---|
MG-132 | ADC proteins are degraded by ubiquitin-dependent mechanisms carried out by 26S proteasome, the major degradation pathway for soluble proteins. Experiments carry out with ADC-transgenic cultures of Trypanosoma cruzi incubated with the peptide aldehyde proteasome inhibitor MG-132 show a marked increase of ADC activity probably due to the reduction of the enzyme turnover rate as shown after blocking protein synthesis with cycloheximide. | Avena sativa |
Cloned (Comment) | Organism |
---|---|
oat enzyme is expressed in Trypanosoma cruzi epimastigotes after transfection with a C-terminal six-His tag and as untagged enzyme, active ADC is expressed in the parasites and the primary translational product is cleaved into 2 polypeptides through a proteolytic process. When the his-tag is added to the C-terminal end of ADC it causes very significant changes on the metabolic stability and catalytic parameters of the heterologous enzyme expressed in the transformed parasites. | Avena sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-difluoromethylarginine | - |
Avena sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-arginine | untagged ADC | Avena sativa | |
0.31 | - |
L-arginine | His-tagged ADC | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis | Avena sativa |
42000 | - |
1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis | Avena sativa |
66000 | - |
inactive precursor protein, active recombinant ADC is formed in vivo by a complex of two His-tagged polypeptide chains of 42 and 24 kDa by a subsequent incubation during different times with extracts of Arabidopsis seedlings which contain a proteolytic activity, processing of oat ADC does not require a specific protease | Avena sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Avena sativa | first reaction of a putrescine synthetic pathway found in bacteria and plants | agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | P22220 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | post-translational proteolysis of ADC depends on the presence of a protease-sensitive loop in the structure of the protein | Avena sativa |
Purification (Comment) | Organism |
---|---|
ADC protein attached to the six-His tag at the C-terminal end is purified from parasite extracts by immobilized metal affinity chromatography through a Ni2+-agarose column | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activities of extracts obtained from ADC or ADCH-transfected parasites are higher in the presence of oxidized glutathione than under reducing conditions with dithiothreitol in the reaction mixture | Avena sativa |
0.0012 | - |
untagged ADC | Avena sativa |
0.0331 | - |
His-tagged ADC | Avena sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | first reaction of a putrescine synthetic pathway found in bacteria and plants | Avena sativa | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis | Avena sativa |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Avena sativa |
arginine decarboxylase | - |
Avena sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Avena sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | To ascertain whether the ADC enzyme expressed in transgenic Trypanosoma cruzi has a modified metabolic turnover when attached to the His-tag, cycloheximide is added to parasite cultures transformed with plasmids to stop protein synthesis. ADC half-life inside the parasite is 150 min, the enzyme attached to the His-tail is more stable, with a half-life of about 14 h. | Avena sativa | |
0.9 | - |
L-arginine | untagged ADC | Avena sativa | |
9.9 | - |
L-arginine | His-tagged ADC | Avena sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | ADC belongs to the group IV pyridoxal 5'-phosphate-dependent decarboxylases | Avena sativa |