Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-Norpyridoxal phosphate | reactivation of the apoenzyme | Escherichia coli | |
6-Methylpyridoxal phosphate | reactivation of the apoenzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Nor-2-butylpyridoxal phosphate | - |
Escherichia coli | |
5-Deoxypyridoxal | - |
Escherichia coli | |
agmatine | - |
Escherichia coli | |
beta-(2-Methyl-3-hydroxy-4-formylpyridine-5)-propionic acid | - |
Escherichia coli | |
citrulline | - |
Escherichia coli | |
guanidine | - |
Escherichia coli | |
Guanidinoacetic acid | - |
Escherichia coli | |
L-2-Amino-3-guanidinopropanoate | - |
Escherichia coli | |
L-2-amino-5-guanidinopentanoate | - |
Escherichia coli | |
L-2-amino-6-guanidinohexanoate | - |
Escherichia coli | |
L-2-Chloro-5-guanidinopentanoate | - |
Escherichia coli | |
L-2-Hydroxy-5-guanidinopentanoate | - |
Escherichia coli | |
L-Lys | - |
Escherichia coli | |
methylguanidine | - |
Escherichia coli | |
Orn | L-Orn | Escherichia coli | |
pyridoxal | - |
Escherichia coli | |
spermidine | - |
Escherichia coli | |
Urea | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
L-Arg | - |
Escherichia coli | |
1.2 | - |
L-canavanine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
350 | 370 | - |
Escherichia coli |
410 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Escherichia coli | agmatine + CO2 | - |
? | |
L-arginine | - |
Escherichia coli B / ATCC 11303 | agmatine + CO2 | - |
? | |
L-Canavanine | - |
Escherichia coli | ? | - |
? | |
L-Canavanine | - |
Escherichia coli B / ATCC 11303 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 40% of maximal activity, 50°C: about 70% of maximal activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | pH 3.5: about 50% of maximal activity, pH 7.0: 23% of maximal activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a formyl group of pyridoxal 5'-phosphate is bound to the 6-amino group of a Lys residue. Binds 10 mol of pyridoxal 5'-phosphate per mol of enzyme | Escherichia coli |