Cloned (Comment) | Organism |
---|---|
gene ldcC, recombinant overexpression in Escherichia coli strain BL21(DE3) in inclusion bodies with 3-10 mg/ml protein in batch cultivation. The aggregated enzyme EcLDCc-CatIBs is catalytically active | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of an innovative immobilisation approach using catalytically active recombinant constitutive L-lysine decarboxylase (EcLDCc) in inclusion bodies, CatIBs, overview. EcLDCc-CatIBs can compete with the whole cell biocatalyst in production of cadaverine | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Escherichia coli | - |
cadaverine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P52095 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Escherichia coli | cadaverine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodecamer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
constitutive LDCc | - |
Escherichia coli |
EcLDCc | - |
Escherichia coli |
L-lysine decarboxylase | - |
Escherichia coli |
LDC | - |
Escherichia coli |
ldcC | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8 | broad optimum, soluble wild-type enzyme | Escherichia coli |
8 | 8.5 | recombinant enzyme inclusion bodies, EcLDCc-CatIBs, the activity is dependent on the medium | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.8 | soluble wild-type enzyme, maximal activity at pH 6.2-8.0, 30% of maximal activity at pH 8.8 | Escherichia coli |
7.5 | 9 | recombinant enzyme inclusion bodies, EcLDCc-CatIBs, range of higher activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the L-lysine decarboxylase (LDC) genes from Escherichia coli include genes cadA and ldcC encoding the acid-inducible enzyme CadA and the constitutive LDCc, respectively | Escherichia coli |