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Literature summary for 4.1.1.18 extracted from

  • Park, S.H.; Soetyono, F.; Kim, H.K.
    Cadaverine production by using cross-linked enzyme aggregate of Escherichia coli lysine decarboxylase (2017), J. Microbiol. Biotechnol., 27, 289-296 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the immobilized recombinant enzyme CadACLEA can be used as a potential catalyst for efficient production of cadaverine Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene cadA, recombinant enzyme expression in Escherichia coli strain XL-1 Blue Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the recombinant enzyme CadA, preparation of a cross-linked enzyme aggregate (CLEA) of Escherichia coli CadA and bioconversion of lysine using CadACLEA. The thermostability of CadACLEA is significantly higher than that of CadAfree. The optimum temperatures of CadAfree and CadACLEA are 60°C and 55°C, respectively. The thermostability of CadACLEA is significantly higher than that of CadAfree. The optimum pH of both enzymes is 6.0. CadAfree cannot be recovered after use, whereas CadACLEA is rapidly recovered and the residual activity is 53% after the 10th recycle Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine Escherichia coli
-
cadaverine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9H3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.76
-
purified recombinant immobilized enzyme, pH 6.0, 37°C Escherichia coli
21.9
-
purified recombinant free enzyme, pH 6.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
Escherichia coli cadaverine + CO2
-
?

Synonyms

Synonyms Comment Organism
CadA
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
recombinant immobilized enzyme CadACLEA Escherichia coli
60
-
recombinant free enzyme CadAfree Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity profiles of recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the recombinant immobilized enzyme CadACLEA and the recombinant free enzyme CadAfree show over 80% remaining activity after 5 h, pH 6.0 Escherichia coli
37
-
the recombinant immobilized enzyme CadACLEA shows about 60% remaining activity, and the recombinant free enzyme CadAfree shows below 10% remaining activity after 5 h, pH 6.0 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree, stable up to, 30 min, 37°C, inactivation above Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli