Application | Comment | Organism |
---|---|---|
synthesis | the immobilized recombinant enzyme CadACLEA can be used as a potential catalyst for efficient production of cadaverine | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene cadA, recombinant enzyme expression in Escherichia coli strain XL-1 Blue | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the recombinant enzyme CadA, preparation of a cross-linked enzyme aggregate (CLEA) of Escherichia coli CadA and bioconversion of lysine using CadACLEA. The thermostability of CadACLEA is significantly higher than that of CadAfree. The optimum temperatures of CadAfree and CadACLEA are 60°C and 55°C, respectively. The thermostability of CadACLEA is significantly higher than that of CadAfree. The optimum pH of both enzymes is 6.0. CadAfree cannot be recovered after use, whereas CadACLEA is rapidly recovered and the residual activity is 53% after the 10th recycle | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Escherichia coli | - |
cadaverine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9H3 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.76 | - |
purified recombinant immobilized enzyme, pH 6.0, 37°C | Escherichia coli |
21.9 | - |
purified recombinant free enzyme, pH 6.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Escherichia coli | cadaverine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CadA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant immobilized enzyme CadACLEA | Escherichia coli |
60 | - |
recombinant free enzyme CadAfree | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity profiles of recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the recombinant immobilized enzyme CadACLEA and the recombinant free enzyme CadAfree show over 80% remaining activity after 5 h, pH 6.0 | Escherichia coli |
37 | - |
the recombinant immobilized enzyme CadACLEA shows about 60% remaining activity, and the recombinant free enzyme CadAfree shows below 10% remaining activity after 5 h, pH 6.0 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant immobilized enzyme CadACLEA and recombinant free enzyme CadAfree, stable up to, 30 min, 37°C, inactivation above | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |