Literature summary for 4.1.1.18 extracted from

Kim, J.H.; Kim, H.J.; Kim, Y.H.; Jeon, J.M.; Song, H.S.; Kim, J.; No, S.Y.; Shin, J.H.; Choi, K.Y.; Park, K.M.; Yang, Y.H.
Functional study of lysine decarboxylases from Klebsiella pneumoniae in Escherichia coli and application of whole cell bioconversion for cadaverine production (2016), J. Microbiol. Biotechnol., 26, 1586-1592 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene cadA, sequence comparisons and phylogenetic analysis, recombinant functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), the activity of purified CadA from Klebsiella pneumoniae is lower than that of CadA from Escherichia coli, the activity of Klebsiella pneumoniae CadA in whole-cell bioconversion is comparable to that of Escherichia coli CadA, resulting in 90% lysine conversion of L-lysine to cadaverine with cofactor pyridoxal 5'-phosphate	Klebsiella aerogenes
gene ldcC, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Klebsiella aerogenes

Cloned (Comment)

Organism

gene cadA, sequence comparisons and phylogenetic analysis, recombinant functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), the activity of purified CadA from Klebsiella pneumoniae is lower than that of CadA from Escherichia coli, the activity of Klebsiella pneumoniae CadA in whole-cell bioconversion is comparable to that of Escherichia coli CadA, resulting in 90% lysine conversion of L-lysine to cadaverine with cofactor pyridoxal 5'-phosphate

Klebsiella aerogenes

gene ldcC, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Klebsiella aerogenes

Protein Variants

Protein Variants	Comment	Organism
additional information	whole-cell bioconversion by Klebsiella pneumoniae lysine decarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decaarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	Klebsiella aerogenes
additional information	whole-cell bioconversion by Klebsiella pneumoniae lysine decarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	Klebsiella aerogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
7.7	-	L-lysine	pH 6.0, 37°C, recombinant enzyme	Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
L-lysine	Klebsiella aerogenes	-	cadaverine + CO2	-	?
L-lysine	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	-	cadaverine + CO2	-	?
additional information	Klebsiella aerogenes	whole-cell bioconversion by Klebsiella pneumoniae lysine decaarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	?	-	?
additional information	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	whole-cell bioconversion by Klebsiella pneumoniae lysine decaarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Klebsiella aerogenes	A0A094XEM2	-	-
Klebsiella aerogenes	A0A0H3FP92	i.e. Enterobacter aerogenes	-
Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	A0A0H3FP92	i.e. Enterobacter aerogenes	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-lysine	-	Klebsiella aerogenes	cadaverine + CO2	-	?
L-lysine	optimal at 0.25 M lysine	Klebsiella aerogenes	cadaverine + CO2	-	?
L-lysine	-	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	cadaverine + CO2	-	?
L-lysine	optimal at 0.25 M lysine	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	cadaverine + CO2	-	?
additional information	whole-cell bioconversion by Klebsiella pneumoniae lysine decaarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	Klebsiella aerogenes	?	-	?
additional information	enzyme CadA is very substrate specific and shows only very low activity with ornithine and no activity with arginine and diaminopimelate	Klebsiella aerogenes	?	-	?
additional information	enzyme LdcC is very substrate specific and shows only very low activity with ornithine and no activity with arginine and diaminopimelate	Klebsiella aerogenes	?	-	?
additional information	whole-cell bioconversion by Klebsiella pneumoniae lysine decaarboxylase LdcC is markedly lower than that of Klebsiella pneumoniae lysine decarboxylase CadA in Klebsiella pneumoniae cells and in transformed Escherichia coli cells	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	?	-	?
additional information	enzyme LdcC is very substrate specific and shows only very low activity with ornithine and no activity with arginine and diaminopimelate	Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006	?	-	?

Synonyms

Synonyms	Comment	Organism
CadA	-	Klebsiella aerogenes
ldcC	-	Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	recombinant enzyme	Klebsiella aerogenes

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	recombinant enzyme, over 50% of maximal activity within this range, profile overview	Klebsiella aerogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.98	-	L-lysine	pH 6.0, 37°C, recombinant enzyme	Klebsiella aerogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	recombinant enzyme	Klebsiella aerogenes

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Klebsiella aerogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.12	-	L-lysine	pH 6.0, 37°C, recombinant enzyme	Klebsiella aerogenes