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Literature summary for 4.1.1.18 extracted from
- A Liquid-based colorimetric assay of lysine decarboxylase and its application to enzymatic assay (2015), J. Microbiol. Biotechnol., 25, 2110-2115 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ldcC, recombinant expression in Escherichia coli strain BL21(DE3), optimization of conditions for enzyme overexpression, e.g. with respect to pH, cadaverine concentration, and IPTG concentration, overview | Burkholderia thailandensis |
| gene ldcC, recombinant expression in Escherichia coli strain BL21(DE3), optimization of conditions for enzyme overexpression, e.g. with respect to pH, cadaverine concentration, and IPTG concentration, overview | Escherichia coli |
| gene ldcC, recombinant expression in Escherichia coli strain BL21(DE3), optimization of conditions for enzyme overexpression, e.g. with respect to pH, cadaverine concentration, and IPTG concentration, overview | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | Burkholderia thailandensis | - |
cadaverine + CO2 | - |
? |  |
| L-lysine | Escherichia coli | - |
cadaverine + CO2 | - |
? | |
| L-lysine | Klebsiella aerogenes | - |
cadaverine + CO2 | - |
? | |
| L-lysine | Escherichia coli K-12 / MG1655 | - |
cadaverine + CO2 | - |
? | |
| L-lysine | Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 | - |
cadaverine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia thailandensis | - |
- |
- |
| Escherichia coli | P52095 | - |
- |
| Escherichia coli K-12 / MG1655 | P52095 | - |
- |
| Klebsiella aerogenes | A0A0H3FP92 | i.e. Enterobacter aerogenes | - |
| Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 | A0A0H3FP92 | i.e. Enterobacter aerogenes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | - |
Burkholderia thailandensis | cadaverine + CO2 | - |
? | |
| L-lysine | - |
Escherichia coli | cadaverine + CO2 | - |
? | |
| L-lysine | - |
Klebsiella aerogenes | cadaverine + CO2 | - |
? | |
| L-lysine | - |
Escherichia coli K-12 / MG1655 | cadaverine + CO2 | - |
? | |
| L-lysine | - |
Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 | cadaverine + CO2 | - |
? | |
| additional information | evaluation of a simple assay method, a colorimetric assay with pH indicator, applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way, modification of a pH indicator-based assay on solid agar medium | Burkholderia thailandensis | ? | - |
? | |
| additional information | evaluation of a simple assay method, a colorimetric assay with pH indicator, applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way, modification of a pH indicator-based assay on solid agar medium | Escherichia coli | ? | - |
? | |
| additional information | evaluation of a simple assay method, a colorimetric assay with pH indicator, applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way, modification of a pH indicator-based assay on solid agar medium | Klebsiella aerogenes | ? | - |
? | |
| additional information | evaluation of a simple assay method, a colorimetric assay with pH indicator, applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way, modification of a pH indicator-based assay on solid agar medium | Escherichia coli K-12 / MG1655 | ? | - |
? | |
| additional information | evaluation of a simple assay method, a colorimetric assay with pH indicator, applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way, modification of a pH indicator-based assay on solid agar medium | Klebsiella aerogenes ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ldcC | - |
Burkholderia thailandensis |
| ldcC | - |
Escherichia coli |
| ldcC | - |
Klebsiella aerogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Burkholderia thailandensis |
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Burkholderia thailandensis |
| 8 | - |
assay at | Escherichia coli |
| 8 | - |
assay at | Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Burkholderia thailandensis | |
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
| pyridoxal 5'-phosphate | - |
Klebsiella aerogenes | |
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