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Literature summary for 4.1.1.17 extracted from
- Ornithine decarboxylase of Leishmania donovani biochemical properties and possible role of N-terminal extension (2015), Protein Pept. Lett., 22, 130-136 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ODC, sequence comparisons, recombinant expression of His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | removal of the N-terminal extended region of LdODC results in improved stability of the protein, but the truncated enzyme LdODC does not show any activity | Leishmania donovani |
General Stability
| General Stability | Organism |
|---|---|
| after prolong storage, the protein loses its activity | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine | Leishmania donovani | - |
putrescine + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | P27116 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine | - |
Leishmania donovani | putrescine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structural analysis of wild-type and truncated mutant enzymes by circular dichroism and fluorescence spectroscopy | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LdODC | - |
Leishmania donovani |
| ODC | - |
Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Leishmania donovani | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | role of N-terminal extension in the enzyme protein, overview | Leishmania donovani |
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Release 2023.1 (January 2023)
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