Literature summary for 4.1.1.17 extracted from

Dufe, V.T.; Ingner, D.; Heby, O.; Khomutov, A.R.; Persson, L.; Al-Karadaghi, S.
A structural insight into the inhibition of human and Leishmania donovani ornithine decarboxylases by 1-amino-oxy-3-aminopropane (2007), Biochem. J., 405, 261-268.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BLR(DE3)	Leishmania donovani
expression of His6-tagged enzyme in Escherichia coli strain BLR(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged enzyme in complex with inhibitor 1-amino-oxy-3-aminopropane or with both inhibitor and cofactor, sitting drop vapour diffusion method, 15C, 6 mg/ml protein in 25 mM HEPES, pH 7.2, 2 mM DTT, 0.5 mM EDTA, 0.02% Brij 35, 1 mM PMSF and 2 mM 1-amino-oxy-3-aminopropane, with or without 0.02 mM pyridoxal 5'-phosphate, mixed with well solution containing 25% v/v PEG 3350, 0.2 M ammonium acetate and 0.1 M Bis-Tris, pH 6.5, 2 days, needle-cluster crystals, single crystals are obtained by using a well solution containing 18% v/v PEG 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, and 3 mg/ml protein concentration, X-ray diffraction structure determination and analysis at 1.9-3.0 A resolution, molecular replacement	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant His6-tagged enzyme in complex with inhibitor 1-amino-oxy-3-aminopropane or with both inhibitor and cofactor, sitting drop vapour diffusion method, 15C, 6 mg/ml protein in 25 mM HEPES, pH 7.2, 2 mM DTT, 0.5 mM EDTA, 0.02% Brij 35, 1 mM PMSF and 2 mM 1-amino-oxy-3-aminopropane, with or without 0.02 mM pyridoxal 5'-phosphate, mixed with well solution containing 25% v/v PEG 3350, 0.2 M ammonium acetate and 0.1 M Bis-Tris, pH 6.5, 2 days, needle-cluster crystals, single crystals are obtained by using a well solution containing 18% v/v PEG 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, and 3 mg/ml protein concentration, X-ray diffraction structure determination and analysis at 1.9-3.0 A resolution, molecular replacement

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
1-amino-oxy-3-aminopropane	i.e. APA, analysis of the mode of the inhibitor binding to the enzyme, no oxime formation between APA and pyridoxal 5'-phosphate, homology modelling, overview	Homo sapiens
1-amino-oxy-3-aminopropane	i.e. APA, analysis of the mode of the inhibitor binding to the enzyme, no oxime formation between APA and pyridoxal 5'-phosphate, determined from the human enzyme-inhibitor cyrstal structure analysis and homology modelling, overview	Leishmania donovani
alpha-difluoromethylornithine	irreversible inhibitor, a curative agent of West African sleeping sickness	Homo sapiens
alpha-difluoromethylornithine	irreversible inhibitor	Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ornithine	Leishmania donovani	ODC is the first committed enzyme in the polyamine biosynthesis pathway	putrescine + CO2	-	?
L-ornithine	Homo sapiens	ODC is the first committed enzyme in the polyamine biosynthesis pathway	putrescine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P11926	-	-
Leishmania donovani	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-ornithine	-	Leishmania donovani	putrescine + CO2	-	?
L-ornithine	-	Homo sapiens	putrescine + CO2	-	?
L-ornithine	ODC is the first committed enzyme in the polyamine biosynthesis pathway	Leishmania donovani	putrescine + CO2	-	?
L-ornithine	ODC is the first committed enzyme in the polyamine biosynthesis pathway	Homo sapiens	putrescine + CO2	-	?

Synonyms

Synonyms	Comment	Organism
ODC	-	Leishmania donovani
ODC	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Leishmania donovani
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Leishmania donovani
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Leishmania donovani
pyridoxal 5'-phosphate	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000001	-	1-amino-oxy-3-aminopropane	pH 7.5, 37°C, recombinant enzyme	Leishmania donovani
0.000001	-	1-amino-oxy-3-aminopropane	pH 7.5, 37°C, recombinant enzyme	Homo sapiens