Crystallization (Comment) | Organism |
---|---|
co-crystallization with D-ornithine and geneticin sulfate, 23 mg/ml ODC in 10 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 0.5 mM EDTA, 0.01% Brij-20, is preincubated with 25 mM D-ornithine and 100 mM geneticin sulfate for 10 min at room temperature, equal volumes of preincubated enzyme and well solution consisting of 20% polyethylene glycol 3350, 100 mM HEPES, pH 8.0, 10 mM dithiothreitol, 25 mM D-ornithine, pH 7.5, and 100 mM geneticine are used to form crystal drops, crystals diffract to 2.5 A | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
geneticin | G418, weak non-competitive inhibition cs. L-ornithine | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Trypanosoma brucei | first step in polyamine biosynthesis | putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Trypanosoma brucei | putrescine + CO2 | - |
? | |
L-ornithine | first step in polyamine biosynthesis | Trypanosoma brucei | putrescine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
geneticin | pH 8.0 | Trypanosoma brucei | |
8 | - |
geneticin | pH 7.0 | Trypanosoma brucei |