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Literature summary for 4.1.1.17 extracted from

  • Koromilas, A.E.; Kyriakidis, D.A.
    Purification and properties of ornithine decarboxylase from germinated barley seeds (1988), Phytochemistry, 27, 989-992.
No PubMed abstract available
Search for more literature for 4.1.1.17

General Stability

General Stability Organism
50% loss of activity after freezing and thawing of the purified enzyme Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 0.8 mM, 50% inhibition Hordeum vulgare
Ca2+ 0.9 mM, 50% inhibition Hordeum vulgare
Co2+ 0.5 mM, 50% inhibition Hordeum vulgare
K+ 220 mM, 50% inhibition Hordeum vulgare
Mg2+ 15 mM, 50% inhibition Hordeum vulgare
Mn2+ 1.0 mM, 50% inhibition Hordeum vulgare
Na+ 300 mM, 50% inhibition Hordeum vulgare
putrescine
-
 Hordeum vulgare
spermidine
-
 Hordeum vulgare
spermine
-
 Hordeum vulgare
Zn2+ 0.8 mM, 50% inhibition Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 L-Orn
-
 Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin bound Hordeum vulgare 785
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration in presence of 0.25 NaCl Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
-
 Hordeum vulgare

Storage Stability

Storage Stability Organism
0-4°C, 24 h, stable in presence of 0.05 mM pyridoxal 5'-phosphate, 2.5 mM dithiothreitol or 5 mM 2-mercaptoethanol, 10% w/v glycerol at pH 8.5 Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Orn
-
 Hordeum vulgare Putrescine + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Hordeum vulgare

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 Co2+
-
 Hordeum vulgare
0.8
-
 Zn2+
-
 Hordeum vulgare
0.8
-
 Ba2+
-
 Hordeum vulgare
0.9
-
 Ca2+
-
 Hordeum vulgare
1
-
 Mn2+
-
 Hordeum vulgare
15
-
 Mg2+
-
 Hordeum vulgare
220
-
 K+
-
 Hordeum vulgare
300
-
 Na+
-
 Hordeum vulgare