Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asiaticoside | stimulates at 0.0104 mM in vitro by 22%. No stimulation is observed in the absence of pyridoxal 5'-phosphate, so it is unlikely that asiaticoside acts as a PLP mimic | Homo sapiens | |
bilobalide | stimulates at 0.0306 mM in vitro by 18% | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene GAD65, recombinant expression of GST-tagged or MBP-fusion wild-type enzyme, a 1757-bp hGAD65 insert from the pET-5C plasmid subcloned into the pMAL-c2X protein fusion system, in Escherichia coli strain DH5alpha, 1.6fold higher hGAD65 activity of the purified MBP-tagged enzyme construct compared to GST-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Mercaptopropionic acid | - |
Homo sapiens | |
allylglycine | - |
Homo sapiens | |
betulinic acid | - |
Homo sapiens | |
additional information | ginsenosides in ginseng extract, high content of Rb1, inhibit the enzyme | Homo sapiens | |
ursolic acid | - |
Homo sapiens | |
valerenic acid | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Homo sapiens | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q05329 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, recombinant MBP-tagged enzyme from Escherichia coli strain DH5alpha by amylose affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00024 | - |
recombinant GST-tagged enzyme, pH 8.0, 37°C | Homo sapiens |
0.00038 | - |
recombinant MBP-tagged enzyme, pH 8.0, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Homo sapiens | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD65 | - |
Homo sapiens |
glutamic acid decarboxylase 65 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0123 | - |
recombinant MBP-tagged enzyme, pH 8.0, 37°C | Homo sapiens | 3-Mercaptopropionic acid |
General Information | Comment | Organism |
---|---|---|
malfunction | disturbances in GABA levels are responsible for a host of neurologic diseases. A reduction in GABA is also implicated in certain anxiety states such as panic disorder (PD). People suffering from PD have 22% lower GABA levels in the occipital cortex | Homo sapiens |
physiological function | glutamic acid decarboxylase (GAD) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA), a crucial inhibitory neurotransmitter in vertebrate brain. GAD produces GABA from the decarboxylation of glutamic acid. GABA is involved in development and regulation of neuroendocrine function | Homo sapiens |