Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.15 extracted from

  • Tajabadi, N.; Baradaran, A.; Ebrahimpour, A.; Rahim, R.A.; Bakar, F.A.; Manap, M.Y.; Mohammed, A.S.; Saari, N.
    Overexpression and optimization of glutamate decarboxylase in Lactobacillus plantarum Taj-Apis362 for high gamma-aminobutyric acid production (2015), Microb. Biotechnol., 8, 623-632 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.15

Application

Application Comment Organism
food industry the recombinant Lactobacillus plantarum strain overexpressing the Lactobacillus plantarum enzyme can be used as a starter culture for direct incorporation into a food system during fermentation for production of GABA-rich products Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
gene gad, DNA and amino acid sequence determimation and analysis, sequence comparisons, recombinant overexpression in Lactobacillus plantarum strain Taj-Apis362 Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
additional information the optimal fermentation conditions for GABA production from recombinant Lactobacillus plantarum strain overexpressing the Lactobacillus plantarum enzyme using response surface methodology are a glutamic acid concentration of 497.973 mM, temperature 36°C, pH 5.31, and time 60 h. Under these conditions, maximum GABA concentration obtained is 11.09 mM. GAD activity of the cell extract released by the recombinant strain (167.2 units/ml/min) is sevenfold greater than the GAD activity of wild-type cells (23.5 units/ml/min). Modelling of recombinant enzyme production, overview Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Lactiplantibacillus plantarum
-
 4-aminobutanoate + CO2
-
 ?
L-glutamate Lactiplantibacillus plantarum Taj-Apis362
-
 4-aminobutanoate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum W0P1N8
-
-
Lactiplantibacillus plantarum Taj-Apis362 W0P1N8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Lactiplantibacillus plantarum 4-aminobutanoate + CO2
-
 ?
L-glutamate
-
 Lactiplantibacillus plantarum Taj-Apis362 4-aminobutanoate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, recombinant His-tagged enzyme, SDS-PAGE Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
GAD
-
 Lactiplantibacillus plantarum
LbGAD
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Lactiplantibacillus plantarum