Literature summary for 4.1.1.15 extracted from

Lee, K.W.; Shim, J.M.; Yao, Z.; Kim, J.A.; Kim, H.J.; Kim, J.H.
Characterization of a glutamate decarboxylase (GAD) from Enterococcus avium M5 isolated from Jeotgal, a Korean fermented seafood (2017), J. Microbiol. Biotechnol., 27, 1216-1222 .

Search for more literature for 4.1.1.15

Application

Application	Comment	Organism
food industry	Enterococcus avium strain M5 and its gadB gene might be useful when GABA-enriched foods are attempted to be produced, serving as a source of strain and gene	Enterococcus avium

Cloned(Commentary)

Cloned (Comment)	Organism
gene gadB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Enterococcus avium

Inhibitors

Inhibitors	Comment	Organism	Structure
AgNO3	44.4% inhibition at 2 mM	Enterococcus avium
CuSO4	56.8% inhibition at 2 mM	Enterococcus avium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.26	-	L-glutamate	pH 5.5, 45°C, recombinant His-tagged enzyme	Enterococcus avium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CaCl2	activates to 124.3% activity at 2 mM	Enterococcus avium
MnCl2	activates to 116.4% activity at 2 mM	Enterococcus avium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate	Enterococcus avium	-	4-aminobutanoate + CO2	-	?
L-glutamate	Enterococcus avium M5	-	4-aminobutanoate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Enterococcus avium	E0D5D7	isolated from Jeot-gal, a Korean fermented seafood	-
Enterococcus avium M5	E0D5D7	isolated from Jeot-gal, a Korean fermented seafood	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Enterococcus avium

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	strain M5 produces 18.47 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with 3% w/v monosodium L-glutamate. Strain M5 secretes a lot of GABA when grown on MRS medium with monosodium L-glutamate	Enterococcus avium	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	strain M5 produces 18.47 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with 3% w/v monosodium L-glutamate	Enterococcus avium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate	-	Enterococcus avium	4-aminobutanoate + CO2	-	?
L-glutamate	-	Enterococcus avium M5	4-aminobutanoate + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 53755, sequence calculation, x * 53000, recombinant His-tagged enzyme, SDS-PAGE	Enterococcus avium

Synonyms

Synonyms	Comment	Organism
GAD	-	Enterococcus avium
GadB	-	Enterococcus avium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	recombinant His-tagged enzyme	Enterococcus avium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	recombinant His-tagged enzyme	Enterococcus avium

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on, inactive without	Enterococcus avium

pI Value

Organism	Comment	pI Value Maximum	pI Value
Enterococcus avium	sequence calculation	-	5

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Release 2023.1 (January 2023)