Cloned (Comment) | Organism |
---|---|
gene gadB, recombinant expression in GABA aminotransferase-knockout Escherichia coli strain XBT from plasmid pHBP, subcloning in Escherichia coli strain XL-1 Blue | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli, optimization of GABA production method, overview. The highest final GABA concentration, 5.07 g/l, is obtained from 10 g/l of monosodium glutamate with a GABA yield of 83% at 30ºC and pH 3.5. When Pyrococcus horikoshii glutamate decarboxylase is introduced into a GABA aminotransferase knockout Escherichia coli XBT strain, 5.69 g/l of GABA is produced with a GABA yield of 93% | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Pyrococcus horikoshii | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Pyrococcus horikoshii | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Pyrococcus horikoshii |
GadB | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme in Escherichia coli | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
recombinant enzyme in Escherichia coli | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pyrococcus horikoshii |