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Literature summary for 4.1.1.15 extracted from
- Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing gamma-aminobutyrate in Corynebacterium glutamicum (2018), AMB Express, 8, 61 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gadB1, recombinant expression in Corynebacterium glutamicum strain SH, subcloning in Escherichia coli strain JM109 | Escherichia coli |
| gene gadB2, recombinant expression in Corynebacterium glutamicum strain SH, subcloning in Escherichia coli strain JM109. Construction of gadB2 expression strains under different RBS sequence and promoter, expression of gadB2 with bicistronic expression cassette, overview | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of gadB2 produced more GABA in Corynebacterium glutamicum than expression of gadB1. Construction of gadB2 expression strains under different RBS sequence and promoters (tacM, sglB, hmp, ilvE, cg1417, gapA, dtsR, tuf, cspB, odhL, uspA, gdh, sod, and pqo), engineering of RBS sequence and promoter, evaluation of GABA production, overview. In recombinant Corynebacterium glutamicum, the optimal pH for cell growth and Glu biosynthesis is about 7.0, whereas that for GAD activity and conversion of Glu to GABA is 5.0-6.0 | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Escherichia coli | - |
4-aminobutanoate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A0A0J2E2M6 | - |
- |
| Escherichia coli | A0A2Y0HLE8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Escherichia coli | 4-aminobutanoate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Escherichia coli |
| GADB1 | - |
Escherichia coli |
| gadB2 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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