Any feedback?
Literature summary for 4.1.1.15 extracted from
- Purification, properties and cDNA cloning of glutamate decarboxylase in germinated faba bean (Vicia faba L.) (2013), Food Chem., 138, 1945-1951.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gad, cloning from germinated faba bean, DNA and amino acid sequence determination and analysis, sequence comparisons | Vicia faba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Vicia faba |  |
| Aminoxyacetate | - |
Vicia faba | |
| Ba2+ | - |
Vicia faba | |
| Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba | |
| Cu2+ | strong inhibition at 3 mM | Vicia faba | |
| EGTA | - |
Vicia faba | |
| Fe3+ | - |
Vicia faba | |
| L-cysteine | - |
Vicia faba | |
| Mg2+ | - |
Vicia faba | |
| Na2EDTA | - |
Vicia faba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.63 | - |
L-glutamate | pH 6.0, 40°C, recombinant enzyme | Vicia faba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57740 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
| 58000 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Vicia faba | - |
4-aminobutanoate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | M1GCP8 | cv. Qidou 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 32fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Vicia faba |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.44 | - |
purified native enzyme, pH 6.0, 40°C | Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Vicia faba | 4-aminobutanoate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Vicia faba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | Vicia faba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range, profile overview, inactivation above | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Vicia faba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile overview | Vicia faba |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vicia faba | sequence calculation | - |
5.41 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
