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Literature summary for 4.1.1.15 extracted from

  • Akama, K.; Takaiwa, F.
    C-terminal extension of rice glutamate decarboxylase (OsGAD2) functions as an autoinhibitory domain and overexpression of a truncated mutant results in the accumulation of extremely high levels of GABA in plant cells (2007), J. Exp. Bot., 58, 2699-2707.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information mutant lacking 31 amino acids at C-terminal extension shows 40fold higher activity than wild-type at physiological pH value. Rice plants overexpressing the mutant enzyme have aberrant phenotypes such as dwarfism, etiolated leaves, and sterility Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q9AR41 isoform GAD2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
wild-type, pH 7.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
17.3
-
wild-type, pH 7.0, 30°C, presence of Ca2+/calmodulin Oryza sativa
197
-
wild-type, pH 6.0, 30°C, presence of Ca2+/calmodulin Oryza sativa
199
-
wild-type, pH 6.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
605
-
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, presence of Ca2+/calmodulin Oryza sativa
641
-
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
685
-
wild-type, pH 5.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
710
-
wild-type, pH 5.0, 30°C, presence of Ca2+/calmodulin Oryza sativa
2719
-
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, presence of Ca2+/calmodulin Oryza sativa
2775
-
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
4670
-
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, absence of Ca2+/calmodulin Oryza sativa
4850
-
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, presence of Ca2+/calmodulin Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Oryza sativa 4-aminobutanoate + CO2
-
?

Subunits

Subunits Comment Organism
More the C-terminal extension of enzyme plays a role as a strong autoinhibitory domain. Truncation causes the enzyme to act constitutively, with higher activity than wild-type both in vitro and in vivo Oryza sativa