Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant lacking 31 amino acids at C-terminal extension shows 40fold higher activity than wild-type at physiological pH value. Rice plants overexpressing the mutant enzyme have aberrant phenotypes such as dwarfism, etiolated leaves, and sterility | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q9AR41 | isoform GAD2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
wild-type, pH 7.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
17.3 | - |
wild-type, pH 7.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
197 | - |
wild-type, pH 6.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
199 | - |
wild-type, pH 6.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
605 | - |
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
641 | - |
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
685 | - |
wild-type, pH 5.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
710 | - |
wild-type, pH 5.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
2719 | - |
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
2775 | - |
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
4670 | - |
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, absence of Ca2+/calmodulin | Oryza sativa |
4850 | - |
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, presence of Ca2+/calmodulin | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Oryza sativa | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal extension of enzyme plays a role as a strong autoinhibitory domain. Truncation causes the enzyme to act constitutively, with higher activity than wild-type both in vitro and in vivo | Oryza sativa |