Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.15 extracted from

  • Jin, H.; Sha, D.; Wei, J.; Davis, K.M.; Wu, H.; Jin, Y.; Wu, J.Y.
    Effect of apocalmodulin on recombinant human brain glutamic acid decarboxylase (2005), J. Neurochem., 92, 739-748.
    View publication on PubMed
Search for more literature for 4.1.1.15

Activating Compound

Activating Compound Comment Organism Structure
apocalmodulin isoform GAD65, up to 60% activation, truncated isoform DAD67, up to 141% activation, no significant activation of GST-GAD67. Activation is due to an increase in affinity for cofactor pyridoxal 5'-phosphate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 L-glutamate isoform GAD67, lacking aminoterminal 70 amino acids, presence of apocalmodulin Homo sapiens
0.69
-
 L-glutamate isoform GAD67, lacking aminoterminal 70 amino acids Homo sapiens
0.69
-
 L-glutamate isoform GAD67, presence of apocalmodulin Homo sapiens
0.71
-
 L-glutamate isoform GAD67 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform GAD65 and GAD67
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Homo sapiens 4-aminobutanoate + CO2
-
 ?

Subunits

Subunits Comment Organism
More both isoform GAD65 and GAD67 in vivo build up a protein complex with apocalmodulin Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km-value 70 nM for isoform GAD67, 70 nM for isoform GAD67 in presence of apocalmodulin, 490 nM isoform GAD67, lacking aminoterminal 70 amino acids, 320 nM for isoform GAD67, lacking aminoterminal 70 amino acids, in presence of apocalmodulin Homo sapiens