Cloned (Comment) | Organism |
---|---|
6*His-tagged isoforms GAD65 and GAD67 expressed in Saccharomyces cerevisiae | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-deoxypyridoxine-5'-phosphate | about 60% inhibition of GAD65 at 3.5 mM, GAD67 is hardly affected | Homo sapiens | |
4'-O-methylpyridoxine-5'-phosphate | decreases activity of GAD65 in unphysiologically high concentrations, about 60% inhibition at 3.5 mM. GAD67 activity is hardly affected | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-Glu | 6*His-tagged GAD67, crude extract | Homo sapiens | |
1.3 | - |
L-Glu | GAD65 after cleavage with protease factor Xa | Homo sapiens | |
1.4 | - |
L-Glu | 6*His-tagged GAD65 | Homo sapiens | |
1.4 | - |
L-Glu | GAD67 after cleavage with protease factor Xa | Homo sapiens | |
2.5 | - |
L-Glu | GAD67-GST fusion protein expressed in Escherichia coli | Homo sapiens | |
2.9 | - |
L-Glu | GAD65-GST fusion protein expressed in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoform GAD65 and GAD67 expressed in Escherichia coli as fusion protein with glutathione-S-transferase and 6*His-tagged isoforms GAD65 and GAD67 expressed in Saccharomyces cerevisiae | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Glu | - |
Homo sapiens | 4-Aminobutanoate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | GAD65-GST fusion protein expressed in Escherichia coli | Homo sapiens |
39 | 43 | 6*His-tagged GAD65 | Homo sapiens |
39 | 43 | GAD65 after cleavage with protease factor Xa | Homo sapiens |
43 | 50 | 6*His-tagged GAD67, crude extract | Homo sapiens |
43 | 50 | GAD67-GST fusion protein expressed in Escherichia coli | Homo sapiens |
45 | 50 | GAD67 after cleavage with protease factor Xa | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
L-Glu | GAD65 after cleavage with protease factor Xa | Homo sapiens | |
1.8 | - |
L-Glu | 6*His-tagged GAD65 | Homo sapiens | |
1.8 | - |
L-Glu | GAD65-GST fusion protein expressed in Escherichia coli | Homo sapiens | |
6.2 | - |
L-Glu | GAD67 after cleavage with protease factor Xa | Homo sapiens | |
7.7 | - |
L-Glu | GAD67-GST fusion protein expressed in Escherichia coli | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activity of GST-GAD54, GAD65 and 6*His-GAD65 is nearly zero without exogenous pyridoxal 5'-phosphate. Activity of GST-GAD67 is 20% without exogenous pyridoxal 5'-phosphate. Activity of GAD67 is 32% without exogenous pyridoxal 5'-phosphate. Activity of 6*His-GAD67 is 30% without exogenous pyridoxal 5'-phosphate | Homo sapiens |