Cloned (Comment) | Organism |
---|---|
a hybrid cDNA is created by fusing a cDNA for amino acids 1-101 of GAD67 to a human cDNA for amino acids 96-585 of GAD65. The recombinant rGAD67/65 protein is expressed in yeast and has equivalent immunoreactivity to mammalian brain GAD with diabetes sera | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a hybrid cDNA is created by fusing a cDNA for amino acids 1-101 of GAD67 to a human cDNA for amino acids 96-585 of GAD65. The recombinant rGAD67/65 protein is expressed in yeast and has equivalent immunoreactivity to mammalian brain GAD with diabetes sera. rGAD67/65 has enzymatic properties similar to that of the mixed isoforms of GAD preparations from mammalian brain | Homo sapiens |
General Stability | Organism |
---|---|
stability is optimal over 3 months, in lyophilized form at 20°C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-Glu | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.37 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 3 months. rGAD67/65 is stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Glu | - |
Homo sapiens | 4-Aminobutanoate + CO2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | efficient non-covalent binding to the cofactor | Homo sapiens |