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Literature summary for 4.1.1.123 extracted from

  • Schühle, K.; Fuchs, G.
    Phenylphosphate carboxylase A new C-C lyase involved in anaerobic phenol metabolism in Thauera aromatica (2004), J. Bacteriol., 186, 4556-4567 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.123

Application

Application Comment Organism
biotechnology the enzyme system may be useful for biotechnical phenol carboxylation Thauera aromatica

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thauera aromatica

Inhibitors

Inhibitors Comment Organism Structure
Dithionite enzyme preparation containing 6 M enzyme is completely inactivated by 0.1 mM dithionite, and the half-life was less than 1 min. Both the net carboxylation and isotope exchange activities are affected in the same way Thauera aromatica
additional information 50 mM 2-mercaptoethanol, 1 mM dithioerythritol, or 0.5 mM titanium(III) citrate (in the presence of excess Mg2+), do not inhibit the enzyme activity Thauera aromatica
O2 the enzyme is strongly inhibited by oxygen. The enzyme is inactivated in a stoichiometric way, and the half-life was less than 1 min. The phenylphosphate carboxylation and the CO2 exchange activities are equally affected Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 Phenyl phosphate 30°C, pH 6.8 Thauera aromatica
1.5
-
 CO2 30°C, pH 6.8 Thauera aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
K+ Km: 14 mM, requires K+ and a divalent metal cation (Mg2+ or Mn2+) Thauera aromatica
Mg2+ Km: 0.1 mM, requires K+ and a divalent metal cation (Mg2+ or Mn2+) Thauera aromatica
Mn2+ Km: 0.09 mM, requires K+ and a divalent metal cation (Mg2+ or Mn2+) Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenyl phosphate + CO2 + H2O Thauera aromatica
-
 4-hydroxybenzoate + phosphate
-
 ?
phenyl phosphate + CO2 + H2O Thauera aromatica DSM 6984
-
 4-hydroxybenzoate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
-
-
Thauera aromatica DSM 6984
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenyl phosphate + CO2 + H2O
-
 Thauera aromatica 4-hydroxybenzoate + phosphate
-
 ?
phenyl phosphate + CO2 + H2O the enzyme also catalyzes the exchange of free CO2 with the carboxyl group of 4-hydroxybenzoate. The 14CO2 exchange reaction catalyzed by the three subunits of the core enzyme (54, 53, and 10 kDa) requires the fully reversible release of CO2 from 4-hydroxybenzoate with formation of a tightly enzyme-bound phenolate intermediate. Carboxylation of phenylphosphate requires in addition the 18-kDa subunit, which is thought to form the same enzyme-bound energized phenolate intermediate from phenylphosphate with virtually irreversible release of phosphate Thauera aromatica 4-hydroxybenzoate + phosphate
-
 ?
phenyl phosphate + CO2 + H2O
-
 Thauera aromatica DSM 6984 4-hydroxybenzoate + phosphate
-
 ?
phenyl phosphate + CO2 + H2O the enzyme also catalyzes the exchange of free CO2 with the carboxyl group of 4-hydroxybenzoate. The 14CO2 exchange reaction catalyzed by the three subunits of the core enzyme (54, 53, and 10 kDa) requires the fully reversible release of CO2 from 4-hydroxybenzoate with formation of a tightly enzyme-bound phenolate intermediate. Carboxylation of phenylphosphate requires in addition the 18-kDa subunit, which is thought to form the same enzyme-bound energized phenolate intermediate from phenylphosphate with virtually irreversible release of phosphate Thauera aromatica DSM 6984 4-hydroxybenzoate + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 54000 + 1 * 53000 + 1 * 18000 + 1 * 10000, SDS-PAGE Thauera aromatica

Synonyms

Synonyms Comment Organism
phenyl phosphate carboxylase
-
 Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Thauera aromatica

pH Range

pH Minimum pH Maximum Comment Organism
6 7.8 pH 6.0: about 50% of maximal activity, pH 7.8: about 50% of maximal activity Thauera aromatica

General Information

General Information Comment Organism
physiological function the enzyme participates in an anaerobic phenol degradation pathway Thauera aromatica