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Literature summary for 4.1.1.12 extracted from

  • Wang, N.C.; Ko, T.P.; Lee, C.Y.
    Inactive S298R disassembles the dodecameric L-aspartate 4-decarboxylase into dimers (2008), Biochem. Biophys. Res. Commun., 374, 134-137.
    View publication on PubMed
Search for more literature for 4.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
P257H the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) Pseudomonas sp.
S298R the mutation disassembles the dodecameric L-aspartate 4-decarboxylase into inactive dimers (0.1% of the wild type enzymatic activity) Pseudomonas sp.
Y207H the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 strain ATCC 19121
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 mutant enzyme P257H, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
0.02
-
 mutant enzyme S298R, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
0.02
-
 mutant enzyme Y207H, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
20.3
-
 wild type enzyme, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp
-
 Pseudomonas sp. L-Ala + CO2
-
 ?

Subunits

Subunits Comment Organism
dodecamer at pH 5.0, X-ray crystallography Pseudomonas sp.

Synonyms

Synonyms Comment Organism
Asd
-
 Pseudomonas sp.
L-aspartate 4-decarboxylase
-
 Pseudomonas sp.