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Literature summary for 4.1.1.117 extracted from

  • Cheung, J.; Beasley, F.C.; Liu, S.; Lajoie, G.A.; Heinrichs, D.E.
    Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus (2009), Mol. Microbiol., 74, 594-608 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in reactions containing component substrates L-2,3-diaminopropionic acid, citric acid and 2-oxoglutaric acid. 1,2-diaminoethane is not required, this component arises from the SbnH-dependent decarboxylation of a 2,3-diaminoproprionic acid-containing intermediate Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Staphylococcus aureus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
citryl-L-2,3-diaminopropionic acid Staphylococcus aureus
-
citryl-diaminoethane + CO2
-
?
citryl-L-2,3-diaminopropionic acid Staphylococcus aureus NCTC 8325
-
citryl-diaminoethane + CO2
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q2G1M6
-
-
Staphylococcus aureus NCTC 8325 Q2G1M6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, displays an intense yellow colour Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citryl-L-2,3-diaminopropionic acid
-
Staphylococcus aureus citryl-diaminoethane + CO2
-
?
citryl-L-2,3-diaminopropionic acid
-
Staphylococcus aureus NCTC 8325 citryl-diaminoethane + CO2
-
?

Subunits

Subunits Comment Organism
? x * 45800, calculated from sequence Staphylococcus aureus

Synonyms

Synonyms Comment Organism
sbnH
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Staphylococcus aureus

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus calculated from sequence
-
5.9

General Information

General Information Comment Organism
physiological function enzyme is involved in biosynthesis of staphyloferrin B. In vitro synthesized staphyloferrin B readily promotes the growth of iron-starved Staphylococcus aureus, via the ABC transporter SirABC. The SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in vitro Staphylococcus aureus