Application | Comment | Organism |
---|---|---|
synthesis | the SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in reactions containing component substrates L-2,3-diaminopropionic acid, citric acid and 2-oxoglutaric acid. 1,2-diaminoethane is not required, this component arises from the SbnH-dependent decarboxylation of a 2,3-diaminoproprionic acid-containing intermediate | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Staphylococcus aureus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
citryl-L-2,3-diaminopropionic acid | Staphylococcus aureus | - |
citryl-diaminoethane + CO2 | - |
? | |
citryl-L-2,3-diaminopropionic acid | Staphylococcus aureus NCTC 8325 | - |
citryl-diaminoethane + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q2G1M6 | - |
- |
Staphylococcus aureus NCTC 8325 | Q2G1M6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, displays an intense yellow colour | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citryl-L-2,3-diaminopropionic acid | - |
Staphylococcus aureus | citryl-diaminoethane + CO2 | - |
? | |
citryl-L-2,3-diaminopropionic acid | - |
Staphylococcus aureus NCTC 8325 | citryl-diaminoethane + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45800, calculated from sequence | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
sbnH | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Staphylococcus aureus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus aureus | calculated from sequence | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is involved in biosynthesis of staphyloferrin B. In vitro synthesized staphyloferrin B readily promotes the growth of iron-starved Staphylococcus aureus, via the ABC transporter SirABC. The SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in vitro | Staphylococcus aureus |