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Literature summary for 4.1.1.112 extracted from

  • Repizo, G.D.; Blancato, V.S.; Mortera, P.; Lolkema, J.S.; Magni, C.
    Biochemical and genetic characterization of the Enterococcus faecalis oxaloacetate decarboxylase complex (2013), Appl. Environ. Microbiol., 79, 2882-2890.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of gene oadH as maltose-binding protein fusion protein in Escherichia coli, genes oadA-oadD, expression of recombinant His-tagged subunits in Escherichia coli strain BL21(DE3) Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
additional information oadA, oadB, and oadD deletion mutants are constructed, while an oadH mutant strain cannot be obtained in spite of using different genetic strategies Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane Enterococcus faecalis 5737
-
membrane Ef-B is a membrane-bound subunit, while subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane Enterococcus faecalis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oxaloacetate Enterococcus faecalis
-
Pyruvate + CO2
-
?
Oxaloacetate Enterococcus faecalis JH2-2
-
Pyruvate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
genes oadA, oadB, oadC, and oadD
-
Enterococcus faecalis JH2-2
-
genes oadA, oadB, oadC, and oadD
-

Purification (Commentary)

Purification (Comment) Organism
recombinnat maltose-binding protein fusion protein OadH from Escherichia coli by amylase affinity chromatography, recombinant His-tagged subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
Enterococcus faecalis Pyruvate + CO2
-
?
Oxaloacetate
-
Enterococcus faecalis JH2-2 Pyruvate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer the enzyme is constituted of four subunits: catalytic subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and subunit Ef-H. Subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane. The Ef-H subunit is involved in the cytoplasmic Ef-AHD complex formation. Analysis of subunit interactions and complex formation, overview Enterococcus faecalis

Synonyms

Synonyms Comment Organism
OAD
-
Enterococcus faecalis

General Information

General Information Comment Organism
physiological function presence of the membrane-bound Ef-B subunit is required for full alkalinization of the internal medium of Enterococcus faecalis cells during citrate fermentation Enterococcus faecalis