Cloned (Comment) | Organism |
---|---|
expression of gene oadH as maltose-binding protein fusion protein in Escherichia coli, genes oadA-oadD, expression of recombinant His-tagged subunits in Escherichia coli strain BL21(DE3) | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | oadA, oadB, and oadD deletion mutants are constructed, while an oadH mutant strain cannot be obtained in spite of using different genetic strategies | Enterococcus faecalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane | Enterococcus faecalis | 5737 | - |
membrane | Ef-B is a membrane-bound subunit, while subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane | Enterococcus faecalis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxaloacetate | Enterococcus faecalis | - |
Pyruvate + CO2 | - |
? | |
Oxaloacetate | Enterococcus faecalis JH2-2 | - |
Pyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
genes oadA, oadB, oadC, and oadD | - |
Enterococcus faecalis JH2-2 | - |
genes oadA, oadB, oadC, and oadD | - |
Purification (Comment) | Organism |
---|---|
recombinnat maltose-binding protein fusion protein OadH from Escherichia coli by amylase affinity chromatography, recombinant His-tagged subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxaloacetate | - |
Enterococcus faecalis | Pyruvate + CO2 | - |
? | |
Oxaloacetate | - |
Enterococcus faecalis JH2-2 | Pyruvate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the enzyme is constituted of four subunits: catalytic subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and subunit Ef-H. Subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane. The Ef-H subunit is involved in the cytoplasmic Ef-AHD complex formation. Analysis of subunit interactions and complex formation, overview | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
OAD | - |
Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
physiological function | presence of the membrane-bound Ef-B subunit is required for full alkalinization of the internal medium of Enterococcus faecalis cells during citrate fermentation | Enterococcus faecalis |