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Literature summary for 4.1.1.112 extracted from

  • Espariz, M.; Repizo, G.; Blancato, V.; Mortera, P.; Alarcon, S.; Magni, C.
    Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis (2011), FEBS J., 278, 2140-2151.
    View publication on PubMed
Search for more literature for 4.1.1.112

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
ADP 78% residual activity at 0.25 mM Enterococcus faecalis
ATP 37% residual activity at 1 mM Enterococcus faecalis
citrate 74% residual activity at 2 mM Enterococcus faecalis
EDTA complete inhibition at 2 mM Enterococcus faecalis
malate 65% residual activity at 2 mM Enterococcus faecalis
malonate 26% residual activity at 2 mM Enterococcus faecalis
additional information not inhibited by succinate Enterococcus faecalis
NAD+ 40% residual activity at 0.25 mM Enterococcus faecalis
NADH 53% residual activity at 0.25 mM Enterococcus faecalis
oxalate 14% residual activity at 2 mM Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 oxaloacetate calculated value at pH 4.5, temperature not specified in the publication Enterococcus faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+ Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q82YW6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-bounded affinity column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
 Enterococcus faecalis Pyruvate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
CitM
-
 Enterococcus faecalis
OAD
-
 Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.2
-
 oxaloacetate calculated value at pH 4.5, temperature not specified in the publication Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Enterococcus faecalis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5
-
 Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
additional information no nicotinamide cofactors required Enterococcus faecalis

General Information

General Information Comment Organism
malfunction the citrate fermentation phenotype is not affected by citM deletion Enterococcus faecalis
metabolism CitM is not required for efficient citrate utilization Enterococcus faecalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22.3
-
 oxaloacetate calculated value at pH 4.5, temperature not specified in the publication Enterococcus faecalis