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Literature summary for 4.1.1.112 extracted from

  • Benziman, M.; Russo, A.; Hochmann, S.; Weinhouse, H.
    Purification and regulatory properties of the oxaloacetate decarboxylase of Acetobacter xylinum (1978), J. Bacteriol., 134, 1-9.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.112

Inhibitors

Inhibitors Comment Organism Structure
acetate competitive for oxaloacetate, Ki: 12 mM Komagataeibacter xylinus
EDTA inhibition of residual activity without divalent metal ions Komagataeibacter xylinus
L-malate competitive for oxaloacetate, Ki: 4.5 mM Komagataeibacter xylinus
Mn2+ at concentrations higher than 0.4 mM Komagataeibacter xylinus
p-hydroxymercuribenzoate complete inactivation after preincubation at 0.2 mM Komagataeibacter xylinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information positive cooperativity for oxaloacetate Komagataeibacter xylinus
additional information
-
 additional information kinetic data Komagataeibacter xylinus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Komagataeibacter xylinus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 1.2 mM, can be substituted by Mn2+ Komagataeibacter xylinus
Mn2+ can be substituted by Mg2+, Km: 0.08 mM Komagataeibacter xylinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate Komagataeibacter xylinus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Komagataeibacter xylinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Komagataeibacter xylinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Komagataeibacter xylinus
360
-
-
 Komagataeibacter xylinus

Storage Stability

Storage Stability Organism
4°C, glycerol, 2 months Komagataeibacter xylinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
 Komagataeibacter xylinus Pyruvate + CO2
-
 ?
oxaloacetate
-
 Komagataeibacter xylinus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 enzyme assay at Komagataeibacter xylinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 enzyme assay at Komagataeibacter xylinus

Cofactor

Cofactor Comment Organism Structure
NAD+ reaction enhancement, allosteric effector Komagataeibacter xylinus
NADP+ reaction enhancement Komagataeibacter xylinus