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Literature summary for 4.1.1.11 extracted from

  • Monteiro, D.C.; Rugen, M.D.; Shepherd, D.; Nozaki, S.; Niki, H.; Webb, M.E.
    Formation of a heterooctameric complex between aspartate alpha-decarboxylase and its cognate activating factor, PanZ, is CoA-dependent (2012), Biochem. Biophys. Res. Commun., 426, 350-355.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA acetyl-CoA is required for activation Escherichia coli
PanZ analysis of the protein complex between Escherichia coli PanD and PanZ in vitro using isothermal titration calorimetry and mass spectrometry and thereby determine the stoichiometry and affinity of the components of this protein complex, high affinity 4:4 heterooctameric complex, overview. Non-covalent interaction between the acetyl group of acetyl-CoA and the PanD zymogen may be required to induce activation Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene panD, expression of His-tagged enzyme mutant T57V and PanZ in Escherichia coli strain DELTApanD DELTApanZ (DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
T57V site-directed mutagenesis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Escherichia coli
-
beta-alanine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene panD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme mutant T57V and PanZ from Escherichia coli strain DELTApanD DELTApanZ (DE3) by nickel affinity chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
Escherichia coli beta-alanine + CO2
-
?

Synonyms

Synonyms Comment Organism
Aspartate alpha-decarboxylase
-
Escherichia coli

General Information

General Information Comment Organism
additional information regulatory mechanisms for PanD activation and inactivation in vivo, overview Escherichia coli