Application | Comment | Organism |
---|---|---|
pharmacology | Mycobacterium tuberculosis is the etiological agent of tuberculosis and PanD is a potential drug target | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
panD gene, overexpression in Escherichia coli, sequencing | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.219 | - |
L-aspartate | pH 7, 37°C, recombinant enzyme | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant enzyme | Mycobacterium tuberculosis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
4 * 16000, recombinant enzyme, inactive pi-proenzyme, which is cleaved into the 13.22 kDa alpha-subunit and 2.74 kDa beta-subunit, processing is not essential for tetramer formation, SDS-PAGE | Mycobacterium tuberculosis |
64000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Mycobacterium tuberculosis | one of the most crucial steps in the pantothenate synthesis pathway | beta-alanine + CO2 | - |
? | |
L-aspartate | Mycobacterium tuberculosis H37Rv | one of the most crucial steps in the pantothenate synthesis pathway | beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | incubation of panD at 37°C for several hours results in a complete cleavage of the inactive pi-form into the two subunits alpha and beta, optimal cleavage at 37°C for 48 h, cleavage mechanism | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
pH 7, 37°C, recombinant enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Mycobacterium tuberculosis | beta-alanine + CO2 | - |
? | |
L-aspartate | one of the most crucial steps in the pantothenate synthesis pathway | Mycobacterium tuberculosis | beta-alanine + CO2 | - |
? | |
L-aspartate | - |
Mycobacterium tuberculosis H37Rv | beta-alanine + CO2 | - |
? | |
L-aspartate | one of the most crucial steps in the pantothenate synthesis pathway | Mycobacterium tuberculosis H37Rv | beta-alanine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 16000, recombinant enzyme, inactive pi-proenzyme, which is cleaved into the 13.22 kDa alpha-subunit and 2.74 kDa beta-subunit, processing is not essential for tetramer formation, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PanD | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
L-aspartate | pH 7, 37°C, recombinant enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycobacterium tuberculosis |