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Literature summary for 4.1.1.105 extracted from

  • Jadaun, J.S.; Sangwan, N.S.; Narnoliya, L.K.; Tripathi, S.; Sangwan, R.S.
    Withania coagulans tryptophan decarboxylase gene cloning, heterologous expression, and catalytic characteristics of the recombinant enzyme (2017), Protoplasma, 254, 181-192 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene TDC, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Withania coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.49
-
L-tryptophan pH 7.5, 37┬░C, recombinant enzyme Withania coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan Withania coagulans
-
tryptamine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Withania coagulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Withania coagulans

Source Tissue

Source Tissue Comment Organism Textmining
shoot shoot cultures Withania coagulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-tryptophan low activity Withania coagulans 5-hydroxytryptamine + CO2
-
?
L-tryptophan
-
Withania coagulans tryptamine + CO2
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 56380, sequence calculation, 6 * 56000, recombinant His6-tagged enzyme, SDS-PAGE Withania coagulans

Synonyms

Synonyms Comment Organism
TDC
-
Withania coagulans
Tryptophan decarboxylase
-
Withania coagulans
WcTDC
-
Withania coagulans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
recombinant enzyme Withania coagulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.66
-
L-tryptophan pH 7.5, 37┬░C, recombinant enzyme Withania coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
recombinant enzyme Withania coagulans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate kinetics for pyridoxal 5'-phosphate are Km = 0.0058 mM, kcat = 1.46/s Withania coagulans

pI Value

Organism Comment pI Value Maximum pI Value
Withania coagulans sequence calculation
-
8.35

General Information

General Information Comment Organism
additional information three-dimensional structure homology modeling, overview Withania coagulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.114
-
L-tryptophan pH 7.5, 37┬░C, recombinant enzyme Withania coagulans