Literature summary for 4.1.1.105 extracted from

Jadaun, J.S.; Sangwan, N.S.; Narnoliya, L.K.; Tripathi, S.; Sangwan, R.S.
Withania coagulans tryptophan decarboxylase gene cloning, heterologous expression, and catalytic characteristics of the recombinant enzyme (2017), Protoplasma, 254, 181-192 .

Search for more literature for 4.1.1.105

Cloned(Commentary)

Cloned (Comment)	Organism
gene TDC, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Withania coagulans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.49	-	L-tryptophan	pH 7.5, 37°C, recombinant enzyme	Withania coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan	Withania coagulans	-	tryptamine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Withania coagulans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Withania coagulans

Source Tissue

Source Tissue	Comment	Organism	Textmining
shoot	shoot cultures	Withania coagulans	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-hydroxy-L-tryptophan	low activity	Withania coagulans	5-hydroxytryptamine + CO2	-	?
L-tryptophan	-	Withania coagulans	tryptamine + CO2	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 56380, sequence calculation, 6 * 56000, recombinant His6-tagged enzyme, SDS-PAGE	Withania coagulans

Synonyms

Synonyms	Comment	Organism
TDC	-	Withania coagulans
Tryptophan decarboxylase	-	Withania coagulans
WcTDC	-	Withania coagulans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	recombinant enzyme	Withania coagulans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.66	-	L-tryptophan	pH 7.5, 37°C, recombinant enzyme	Withania coagulans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	recombinant enzyme	Withania coagulans

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	kinetics for pyridoxal 5'-phosphate are Km = 0.0058 mM, kcat = 1.46/s	Withania coagulans

pI Value

Organism	Comment	pI Value Maximum	pI Value
Withania coagulans	sequence calculation	-	8.35

General Information

General Information	Comment	Organism
additional information	three-dimensional structure homology modeling, overview	Withania coagulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.114	-	L-tryptophan	pH 7.5, 37°C, recombinant enzyme	Withania coagulans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)