Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli KRX cells | Gelatoporia subvermispora |
gene tdc, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain KRX | Gelatoporia subvermispora |
Protein Variants | Comment | Organism |
---|---|---|
G351A | site-directed saturation mutagenesis, the mutant shows additional activity with phenolic substrates | Gelatoporia subvermispora |
G351I | site-directed saturation mutagenesis, the mutant shows additional activity with phenolic substrates | Gelatoporia subvermispora |
G351L | the mutation adds L-tyrosine and 3,4-dihydroxy-L-phenylalanine decarboxylase activity while retaining stereospecificity and L-tryptophan decarboxylase activity compared to the wild type enzyme | Gelatoporia subvermispora |
G351L | site-directed saturation mutagenesis, the mutant shows additional 3,4-dihydroxy-L-phenylalanine (L-DOPA) decarboxylase activity, and G351L shows approximately twelvefold and fivefold increases in Km values for L-tryptophan and 5-hydroxy-L-tryptophan compared to wild-type, as well as increased values for L-tyrosine and L-DOPA (2.44 and 3.4 mM), respectively | Gelatoporia subvermispora |
G351S | the mutation adds L-tyrosine and 3,4-dihydroxy-L-phenylalanine decarboxylase activity while retaining stereospecificity and L-tryptophan decarboxylase activity compared to the wild type enzyme | Gelatoporia subvermispora |
G351S | site-directed saturation mutagenesis, the mutant shows additional L-tyrosine decarboxylase activity while retaining stereospecificity and L-tryptophan decarboxylase activity | Gelatoporia subvermispora |
G351V | site-directed saturation mutagenesis, the mutant shows additional L-tyrosine decarboxylase activity while retaining stereospecificity and L-tryptophan decarboxylase activity | Gelatoporia subvermispora |
additional information | except for G351T, all engineered variants are opened for phenolic substrates compared to wild-type enzyme. Neither of the engineered enzymes turns over L-phenylalanine, L-histidine, or D-amino acids | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Gelatoporia subvermispora | |
0.16 | - |
L-tryptophan | recombinant wild-type enzyme, pH 7.5, 37°C | Gelatoporia subvermispora | |
0.16 | - |
L-tryptophan | wild type enzyme, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.29 | - |
L-tryptophan | mutant enzyme G351S, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.32 | - |
5-hydroxy-L-tryptophan | recombinant wild-type enzyme, pH 7.5, 37°C | Gelatoporia subvermispora | |
0.32 | - |
5-hydroxy-L-tryptophan | wild type enzyme, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.35 | - |
5-hydroxy-L-tryptophan | mutant enzyme G351S, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
1.72 | - |
5-hydroxy-L-tryptophan | mutant enzyme G351L, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
1.72 | - |
5-hydroxy-L-tryptophan | recombinant mutant G351L, pH 7.5, 37°C | Gelatoporia subvermispora | |
1.94 | - |
L-tryptophan | mutant enzyme G351L, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
1.94 | - |
L-tryptophan | recombinant mutant G351L, pH 7.5, 37°C | Gelatoporia subvermispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-L-tryptophan | Gelatoporia subvermispora | - |
5-hydroxytryptamine + CO2 | - |
? | |
5-hydroxy-L-tryptophan | Gelatoporia subvermispora B | - |
5-hydroxytryptamine + CO2 | - |
? | |
L-tryptophan | Gelatoporia subvermispora | - |
tryptamine + CO2 | - |
? | |
L-tryptophan | Gelatoporia subvermispora B | - |
tryptamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | - |
- |
- |
Gelatoporia subvermispora | M2RF26 | - |
- |
Gelatoporia subvermispora B | M2RF26 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography and Superdex 200 gel filtration | Gelatoporia subvermispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-L-tryptophan | - |
Gelatoporia subvermispora | 5-hydroxytryptamine + CO2 | - |
? | |
5-hydroxy-L-tryptophan | - |
Gelatoporia subvermispora B | 5-hydroxytryptamine + CO2 | - |
? | |
L-tryptophan | - |
Gelatoporia subvermispora | tryptamine + CO2 | - |
? | |
L-tryptophan | - |
Gelatoporia subvermispora B | tryptamine + CO2 | - |
? | |
additional information | the enzyme does not accept L-tyrosine, L-phenylalanine, L-DOPA, or L-histidine, nor does any of the D-configured amino acids lead to a product | Gelatoporia subvermispora | ? | - |
? | |
additional information | the enzyme acts as a decarboxylase that is strictly specific for L-tryptophan and 5-hydroxy-L-tryptophan. No activity of the wild-type enzyme with 1-, 2-, 4-, 5-, 6-, and 7-methyl-L-tryptophan or with L-tyrosine, 3,4-dihydroxy-L-phenylalanine, or L-histidine | Gelatoporia subvermispora | ? | - |
? | |
additional information | the enzyme acts as a decarboxylase that is strictly specific for L-tryptophan and 5-hydroxy-L-tryptophan. No activity of the wild-type enzyme with 1-, 2-, 4-, 5-, 6-, and 7-methyl-L-tryptophan or with L-tyrosine, 3,4-dihydroxy-L-phenylalanine, or L-histidine | Gelatoporia subvermispora B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57600, calculated from amino acid sequence | Gelatoporia subvermispora |
Synonyms | Comment | Organism |
---|---|---|
(5-hydroxy-)L-tryptophan decarboxylase | - |
Gelatoporia subvermispora |
CsTDC | - |
Gelatoporia subvermispora |
L-Tryptophan decarboxylase | - |
Gelatoporia subvermispora |
TDC | - |
Gelatoporia subvermispora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Gelatoporia subvermispora |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
5-hydroxy-L-tryptophan | mutant enzyme G351S, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.17 | - |
5-hydroxy-L-tryptophan | recombinant wild-type enzyme, pH 7.5, 37°C | Gelatoporia subvermispora | |
0.17 | - |
5-hydroxy-L-tryptophan | wild type enzyme, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.26 | - |
5-hydroxy-L-tryptophan | mutant enzyme G351L, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
0.82 | - |
L-tryptophan | mutant enzyme G351S, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
1.21 | - |
L-tryptophan | recombinant wild-type enzyme, pH 7.5, 37°C | Gelatoporia subvermispora | |
1.21 | - |
L-tryptophan | wild type enzyme, at pH 7.5 and 37°C | Gelatoporia subvermispora | |
1.79 | - |
L-tryptophan | mutant enzyme G351L, at pH 7.5 and 37°C | Gelatoporia subvermispora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Gelatoporia subvermispora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Gelatoporia subvermispora |
General Information | Comment | Organism |
---|---|---|
additional information | residue G351 is the key catalytic residue of the enzyme | Gelatoporia subvermispora |