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Literature summary for 4.1.1.102 extracted from
- Isofunctional enzymes PAD1 and UbiX catalyze formation of a novel cofactor required by ferulic acid decarboxylase and 4-hydroxy-3-polyprenylbenzoic acid decarboxylase (2015), ACS Chem. Biol., 10, 1137-1144 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
ferulate | pH 7.0, 25°C | Saccharomyces cerevisiae |  |
| 0.18 | - |
4-coumarate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.18 | - |
trans-cinnamate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 480000 | - |
PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q03034 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumarate | - |
Saccharomyces cerevisiae | 4-vinylphenol + CO2 | - |
? | |
| ferulate | - |
Saccharomyces cerevisiae | 4-vinylguaiacol + CO2 | - |
? | |
| trans-cinnamate | - |
Saccharomyces cerevisiae | styrene + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 57898, mass spectrometry, x * 57898, calculated from sequence, recombinant His-tagged protein | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
ferulate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 3.8 | - |
trans-cinnamate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 4.6 | - |
4-coumarate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | a modified form of FMN acts as cofactor | Saccharomyces cerevisiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | protein PAD1 catalyzes the formation of a diffusible cofactor required by FDC for decarboxylase activity. Escherichia coli protein UbiX can also activate FDC | Saccharomyces cerevisiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.6 | - |
ferulate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 21 | - |
trans-cinnamate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 25.5 | - |
4-coumarate | pH 7.0, 25°C | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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