Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.101 extracted from

  • Arthurs, C.E.; Lloyd, D.
    Kinetics, stereospecificity, and expression of the malolactic enzyme (1999), Appl. Environ. Microbiol., 65, 3360-3363.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.101

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 L-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides
8
-
 DL-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides
8.3
-
 D-malate pH 4.5, 25°C, activity of cell extract Secundilactobacillus collinoides

Organism

Organism UniProt Comment Textmining
Secundilactobacillus collinoides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-malate
-
 Secundilactobacillus collinoides D-lactate + CO2
-
 ?
DL-malate
-
 Secundilactobacillus collinoides DL-lactate + CO2
-
 ?
L-malate
-
 Secundilactobacillus collinoides L-lactate + CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
-
 Secundilactobacillus collinoides

Expression

Organism Comment Expression
Secundilactobacillus collinoides enzyme is constitutively expressed. Addition of L-malic acid (37 mM) to the growth medium results in increased activity, addition of the D-isomer alone or the racemic mixture results in lower activities. Addition of the main sugars in apple juice (fructose, sucrose, and glucose) to the growth medium in the presence of malic acid represses production of the enzyme. In the absence of malic acid, addition of sugars to the growth medium somewhat increases the residual malolactic activity additional information