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Literature summary for 4.1.1.101 extracted from

  • Spettoli, P.; Nuti, M.; Zamorani, A.
    Properties of malolactic activity purified from Leuconostoc oenos ML34 by affinity chromatography (1984), Appl. Environ. Microbiol., 48, 900-901.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.101

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 L-malate pH 5.8, 30°C Oenococcus oeni

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 92% of the activity with Mn2+ Oenococcus oeni
Cu2+ 28% of the activity with Mn2+ Oenococcus oeni
Fe2+ 45% of the activity with Mn2+ Oenococcus oeni
Mg2+ 57% of the activity with Mn2+ Oenococcus oeni
Mn2+ best activator, Km value 0.028 mM Oenococcus oeni
Zn2+ 59% of the activity with Mn2+ Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 gel filtration Oenococcus oeni

Organism

Organism UniProt Comment Textmining
Oenococcus oeni
-
-
-
Oenococcus oeni ML34
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oenococcus oeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-malate
-
 Oenococcus oeni L-lactate + CO2
-
 ?
L-malate
-
 Oenococcus oeni ML34 L-lactate + CO2
-
 ?
additional information no substrate: D-Malic, DL-tartaric, citric acid, and pyruvic acid Oenococcus oeni ?
-
 ?
additional information no substrate: D-Malic, DL-tartaric, citric acid, and pyruvic acid Oenococcus oeni ML34 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Oenococcus oeni

Cofactor

Cofactor Comment Organism Structure
NAD+ Km value 0.13 mM Oenococcus oeni