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Literature summary for 4.1.1.101 extracted from
- Purification and properties of a malolactic enzyme from a strain of Leuconostoc mesenteroides isolated from grapes (1982), Appl. Environ. Microbiol., 43, 357-361.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | competitive | Leuconostoc mesenteroides |  |
| D-fructose-1,6-bisphosphate | noncompetitive | Leuconostoc mesenteroides | |
| D-Tartrate | competitive | Leuconostoc mesenteroides | |
| L-lactate | noncompetitive | Leuconostoc mesenteroides | |
| L-Tartrate | competitive | Leuconostoc mesenteroides | |
| meso-tartrate | competitive | Leuconostoc mesenteroides | |
| oxamate | noncompetitive | Leuconostoc mesenteroides | |
| succinate | competitive | Leuconostoc mesenteroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.7 | - |
L-malate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | allosteric activator, binds to the protein before and independently of malate | Leuconostoc mesenteroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 235000 | - |
gel filtration | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.23 | - |
pH 6.0, 25°C | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-malate | - |
Leuconostoc mesenteroides | L-lactate + CO2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Leuconostoc mesenteroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | Km value 0.045 mM | Leuconostoc mesenteroides | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000011 | - |
citrate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 1.2 | - |
L-Tartrate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 9 | - |
oxamate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 10 | - |
meso-tartrate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 11 | - |
D-Tartrate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 32 | - |
D-fructose-1,6-bisphosphate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 78 | - |
succinate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
| 276 | - |
L-lactate | pH 6.0, 25°C | Leuconostoc mesenteroides | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leuconostoc mesenteroides | isoelectric focusing | - |
4.35 |
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