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Literature summary for 4.1.1.1 extracted from

  • Pohl, M.; Siegert, P.; Mesch, K.; Bruhn, H.; Groetzinger, J.
    Active site mutants of pyruvate decarboxylase from Zymomonas mobilis: A site-directed mutagenesis study of L112, I472, I476, E473, and N482 (1998), Eur. J. Biochem., 257, 538-546.
    View publication on PubMed
Search for more literature for 4.1.1.1

Protein Variants

Protein Variants Comment Organism
E473D inactive Zymomonas mobilis
E473N inactive Zymomonas mobilis
I472A mutation influences the decarboxylation and carboligation reactions. The enlarged substrate-binding site allows the decarboxylation of longer aliphatic 2-keto acids (C4-C6) as well as aromatic 2-keto acids besides pyruvate, yielding hydroxypropiophenone, benzoin and phenylacetylcarbinol. Mutation impairs enantioselectivity Zymomonas mobilis
I476A mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
I476E mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
I476L mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
I476V mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
N482D mutation has a significant influence on the carboligation reaction, the binding of the cofactors and the thermostability are not affected Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 pyruvate wild-type, pH 6.5, 30°C Zymomonas mobilis
1.1
-
 pyruvate mutant L112A, pH 6.5, 30°C Zymomonas mobilis
3.4
-
 pyruvate mutant N482D, pH 6.5, 30°C Zymomonas mobilis
4.7
-
 pyruvate mutant I476L, pH 6.5, 30°C Zymomonas mobilis
6.8
-
 pyruvate mutant I476A, pH 6.5, 30°C Zymomonas mobilis
8.9
-
 pyruvate mutant I476V, pH 6.5, 30°C Zymomonas mobilis
9.1
-
 pyruvate mutant I472A, pH 6.5, 30°C Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Zymomonas mobilis ethanal + CO2
-
 ?
pyruvate + benzaldehyde wild-type 100% conversion, 98% enantiomeric excess, mutant I472A, 60% conversion, 70% enantiomeric excess, mutant I476E, 6% conversion, 60% enantiomeric excess of S-enantiomer Zymomonas mobilis (R)-phenylacetylcarbinol + CO2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 mutant I476E, melting temperature Zymomonas mobilis
52
-
 mutant I476A, melting temperature Zymomonas mobilis
52
-
 mutant N482D, melting temperature Zymomonas mobilis
53
-
 mutant L112A, melting temperature Zymomonas mobilis
58
-
 mutant I476V, melting temperature Zymomonas mobilis
60
-
 mutant I476L, melting temperature Zymomonas mobilis
64
-
 wild-type, melting temperature Zymomonas mobilis