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Literature summary for 4.1.1.1 extracted from

  • Lu, J.; Brigham, C.; Gai, C.; Sinskey, A.
    Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha (2012), Appl. Microbiol. Biotechnol., 96, 283-297.
    View publication on PubMed

Application

Application Comment Organism
synthesis Ralstonia eutropha H16 produces polyhydroxybutanoate as an intracellular carbon storage material. The excess carbon can be redirected in engineered strains from polyhydroxybutanoate storage to the production of isobutanol and 3-methyl-1-butanol (branched-chain higher alcohols). Strains of Ralstonia eutropha with isobutyraldehyde dehydrogenase activity, in combination with the overexpression of plasmid-borne, native branched-chain amino acid biosynthesis pathway genes and the overexpression of heterologous ketoisovalerate decarboxylase gene, are employed for the biosynthesis of isobutanol and 3-methyl-1-butanol. One mutant strain produces over 180 mg/l branched-chain alcohols in flask culture, and is significantly more tolerant of isobutanol toxicity than wild-type. After the elimination of genes ilvE, bkdAB, and aceE, the production titer improves to 270 mg/l isobutanol and 40 mg/l 3-methyl-1-butanol. Under semicontinuous flask cultivation, the strain grows and produces more than 14 g/l branched-chain alcohols over the duration of 50 days Lactococcus lactis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
expression Ralstonia eutropha Lactococcus lactis subsp. lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis subsp. lactis Q684J7
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Synonyms

Synonyms Comment Organism
KivD
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Lactococcus lactis subsp. lactis