Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | dephosphorylation of Pdc1p by alkaline phosphatase inhibits the enzyme activity by 50% | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | 6 | pyruvate | pH 6.0, 37°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Pdc1p is subject to reversible phosphorylation at Ser223 that is dependent on glucose availability, dephosphorylation of Pdc1p by alkaline phosphatase inhibits the enzyme activity by 50%. Phosphorylation of Pdc1p is dependent on the growth phase, being hyperphosphorylated in the logarithmic phase, dependent on the presence of Ser/Thr protein phosphatase SIT4p. The Ser/Thr protein phosphatase SIT4 reduces Pdc1p activity by altering the apparent affinity for the cofactor thiamine pyrophosphate | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Pdc1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required, the Ser/Thr protein phosphatase SIT4 reduces Pdc1p activity by altering the apparent affinity for the cofactor thiamine pyrophosphate | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the Ser/Thr protein phosphatase SIT4 phosphatase decreases the pyruvate decarboxylase activity | Saccharomyces cerevisiae |
metabolism | pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase Sit4p in the yeast Saccharomyces cerevisiae, mechanism of regulation of pyruvate decarboxylase activity, overview | Saccharomyces cerevisiae |
physiological function | the enzyme is essential for directing the glucose flux to ethanol production. The activity of Pdc1p is regulated by phosphorylation of serine residues during growth | Saccharomyces cerevisiae |