Application | Comment | Organism |
---|---|---|
synthesis | Candida utilis PDC is a stable and high productivity enzyme for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor | Cyberlindnera jadinii |
synthesis | PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor | Saccharomyces cerevisiae |
synthesis | PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor | Candida tropicalis |
synthesis | PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor | Kluyveromyces marxianus |
General Stability | Organism |
---|---|
half-life of 3 days for both crude enzyme extract and 2 days for whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C | Kluyveromyces marxianus |
half-life of 5 days for both crude enzyme extract and 3.5 days for whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C | Saccharomyces cerevisiae |
half-life of 7 days for both crude enzyme extract and whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C, half-life of nearly 2 weeks for crude enzyme extract and slightly longer for whole cell biomass in absence of benzaldehyde | Cyberlindnera jadinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cyberlindnera jadinii | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Saccharomyces cerevisiae | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Candida tropicalis | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Kluyveromyces marxianus | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Saccharomyces cerevisiae UNSW 102200 | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Kluyveromyces marxianus UNSW 510700 | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Candida tropicalis LU57 | the enzyme also performs carboligation reactions | ? | - |
? | |
additional information | Cyberlindnera jadinii UNSW 70940 | the enzyme also performs carboligation reactions | ? | - |
? | |
pyruvate | Cyberlindnera jadinii | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Candida tropicalis | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Kluyveromyces marxianus | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Saccharomyces cerevisiae UNSW 102200 | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Kluyveromyces marxianus UNSW 510700 | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Candida tropicalis LU57 | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Cyberlindnera jadinii UNSW 70940 | - |
acetaldehyde + CO2 | - |
? | |
pyruvate + acetaldehyde | Cyberlindnera jadinii | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Saccharomyces cerevisiae | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Candida tropicalis | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Kluyveromyces marxianus | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Saccharomyces cerevisiae UNSW 102200 | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Kluyveromyces marxianus UNSW 510700 | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Candida tropicalis LU57 | - |
acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | Cyberlindnera jadinii UNSW 70940 | - |
acetoin + CO2 | - |
? | |
pyruvate + benzaldehyde | Cyberlindnera jadinii | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Saccharomyces cerevisiae | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Candida tropicalis | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Kluyveromyces marxianus | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Saccharomyces cerevisiae UNSW 102200 | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Kluyveromyces marxianus UNSW 510700 | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Candida tropicalis LU57 | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | Cyberlindnera jadinii UNSW 70940 | stereospecific reaction, optimization of the biotransformation assay method | (R)-phenylacetylcarbinol + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
- |
- |
Candida tropicalis LU57 | - |
- |
- |
Cyberlindnera jadinii | - |
- |
- |
Cyberlindnera jadinii UNSW 70940 | - |
- |
- |
Kluyveromyces marxianus | - |
- |
- |
Kluyveromyces marxianus UNSW 510700 | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae UNSW 102200 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
(R)-phenylacetylcarbinol production rate | Cyberlindnera jadinii |
additional information | - |
(R)-phenylacetylcarbinol production rate | Saccharomyces cerevisiae |
additional information | - |
(R)-phenylacetylcarbinol production rate | Candida tropicalis |
additional information | - |
(R)-phenylacetylcarbinol production rate | Kluyveromyces marxianus |
Storage Stability | Organism |
---|---|
half-life of 3 days for crude enzyme extract and less than 1 day for whole cell biomass in absence or presence of benzaldehyde at 22°C | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also performs carboligation reactions | Cyberlindnera jadinii | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Candida tropicalis | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Kluyveromyces marxianus | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Saccharomyces cerevisiae UNSW 102200 | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Kluyveromyces marxianus UNSW 510700 | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Candida tropicalis LU57 | ? | - |
? | |
additional information | the enzyme also performs carboligation reactions | Cyberlindnera jadinii UNSW 70940 | ? | - |
? | |
pyruvate | - |
Cyberlindnera jadinii | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Candida tropicalis | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Kluyveromyces marxianus | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae UNSW 102200 | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Kluyveromyces marxianus UNSW 510700 | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Candida tropicalis LU57 | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Cyberlindnera jadinii UNSW 70940 | acetaldehyde + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Cyberlindnera jadinii | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Saccharomyces cerevisiae | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Candida tropicalis | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Kluyveromyces marxianus | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Saccharomyces cerevisiae UNSW 102200 | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Kluyveromyces marxianus UNSW 510700 | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Candida tropicalis LU57 | acetoin + CO2 | - |
? | |
pyruvate + acetaldehyde | - |
Cyberlindnera jadinii UNSW 70940 | acetoin + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Cyberlindnera jadinii | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Saccharomyces cerevisiae | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Candida tropicalis | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Kluyveromyces marxianus | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Saccharomyces cerevisiae UNSW 102200 | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Kluyveromyces marxianus UNSW 510700 | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Candida tropicalis LU57 | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, optimization of the biotransformation assay method | Cyberlindnera jadinii UNSW 70940 | (R)-phenylacetylcarbinol + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Cyberlindnera jadinii |
PDC | - |
Saccharomyces cerevisiae |
PDC | - |
Candida tropicalis |
PDC | - |
Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Cyberlindnera jadinii |
30 | - |
in vivo assay at | Saccharomyces cerevisiae |
30 | - |
in vivo assay at | Candida tropicalis |
30 | - |
in vivo assay at | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Cyberlindnera jadinii | |
thiamine diphosphate | - |
Saccharomyces cerevisiae | |
thiamine diphosphate | - |
Candida tropicalis | |
thiamine diphosphate | - |
Kluyveromyces marxianus |