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Literature summary for 4.1.1.1 extracted from
- Purification and characterization of two isozymes of pyruvate decarboxylase from Rhizopus oryzae (2007), Enzyme Microb. Technol., 40, 675-682.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | substrate inhibition at pyruvate concentrations above 20 mM for isoenzyme 1; substrate inhibition at pyruvate concentrations above 40 mM for isoenzyme 2 | Rhizopus arrhizus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the isozyme shows sigmoidal kinetics with a Hill coefficient of 1.8 | Rhizopus arrhizus | |
| 3.9 | - |
pyruvate | pH 6.5, 35C, isozyme 1 | Rhizopus arrhizus | |
| 4.5 | - |
pyruvate | pH 6.5, 35°C, isozyme 2 | Rhizopus arrhizus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highly required | Rhizopus arrhizus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61000, SDS-PAGE, the isozyme is found in two different native forms, dimer and tetramer, with the dimer being the predominant form | Rhizopus arrhizus |
| 120000 | 128000 | dimeric enzyme form, native PAGE | Rhizopus arrhizus |
| 272000 | - |
tetrameric enzyme form, native PAGE | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Rhizopus arrhizus | - |
acetaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | Q8NK64 | isozyme 2, gene pdcB; isozyme 2 encoded by gene pdcB | - |
| Rhizopus arrhizus | Q8NK65 | isozyme 1, gene pdcA; isozyme 1 encoded by gene pdcA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozyme 17fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Rhizopus arrhizus |
| native isozyme 22fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Rhizopus arrhizus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Rhizopus arrhizus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 59.4 | - |
purified native isozymes | Rhizopus arrhizus |
| 75.4 | - |
purified native isozyme | Rhizopus arrhizus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Rhizopus arrhizus | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61000, SDS-PAGE, the isozyme is found in two different native forms, dimer and tetramer, with the dimer being the predominant form | Rhizopus arrhizus |
| tetramer | 2 * 61000, SDS-PAGE, the isozyme is found in two different native forms, dimer and tetramer, with the dimer being the predominant form | Rhizopus arrhizus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDC | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 6.5 | - |
Rhizopus arrhizus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 8 | 16% of maximal activity at pH 8.0 | Rhizopus arrhizus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Rhizopus arrhizus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhizopus arrhizus | isozyme 2, 2D electrophoresis | - |
5.82 |
| Rhizopus arrhizus | isozyme 1, 2D electrophoresis | - |
5.94 |
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