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Literature summary for 4.1.1.1 extracted from

  • Li, H.; Furey, W.; Jordan, F.
    Role of glutamate 91 in information transfer during substrate activation of yeast pyruvate decarboxylase (1999), Biochemistry, 38, 9992-10003.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Pyruvamide activates Saccharomyces cerevisiae
pyruvate substrate activation pathway, the consequences of binding substrate at C221 are propagated to the active site via the pathway H92 to E91 to W412 to G413 to thiamine diphosphate, role of C221 and E91 Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, E91A, E91D and E91Q mutant PDC in Escherichia coli BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
E91A mutant with 30fold reduced specific activity, reduced turnover number and catalytic efficiency, abolished cooperativity, reduced thermal stability, impaired ability to bind the cofactors Saccharomyces cerevisiae
E91D mutant with 5fold reduced specific activity, reduced turnover number and catalytic efficiency, slightly reduced Hill coefficient, reduced thermal stability, impaired ability to bind the cofactors Saccharomyces cerevisiae
E91Q mutant with 4fold reduced specific activity, reduced turnover number and catalytic efficiency, abolished cooperativity, reduced thermal stability, impaired ability to bind the cofactors Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic data, pH-dependence of steady-state kinetic parameters Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cofactor, requirement Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence Saccharomyces cerevisiae
61320
-
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence Saccharomyces cerevisiae
61468
-
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Saccharomyces cerevisiae
-
acetaldehyde + CO2
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type, E91A, E91D and E91Q mutant PDC, expressed in Escherichia coli, mutants are purified as apoenzymes Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
a 2-oxo carboxylate = an aldehyde + CO2 mechanism Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: pyruvamide Saccharomyces cerevisiae ?
-
?
pyruvate
-
Saccharomyces cerevisiae acetaldehyde + CO2
-
?
pyruvate detailed mechanism Saccharomyces cerevisiae acetaldehyde + CO2
-
ir

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
PDC1
-
Saccharomyces cerevisiae
scpdc1
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38.5
-
Tm, E91A mutant PDC Saccharomyces cerevisiae
42.5
-
Tm, E91D mutant PDC Saccharomyces cerevisiae
45.5
-
Tm, E91Q mutant PDC Saccharomyces cerevisiae
59.5
-
Tm, wild-type PDC Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
pyruvate pH 6, 25°C, wild-type PDC Saccharomyces cerevisiae
3.1
-
pyruvate pH 6, 25°C, E91A mutant PDC Saccharomyces cerevisiae
17.5
-
pyruvate pH 6, 25°C, E91D mutant PDC Saccharomyces cerevisiae
20.4
-
pyruvate pH 6, 25°C, E91Q mutant PDC Saccharomyces cerevisiae
73.1
-
pyruvate pH 6, 25°C, wild-type PDC Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate requirement, located at the active site at the interface of the alpha and gamma domains Saccharomyces cerevisiae