Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Pyruvamide | activates | Saccharomyces cerevisiae | |
pyruvate | substrate activation pathway, the consequences of binding substrate at C221 are propagated to the active site via the pathway H92 to E91 to W412 to G413 to thiamine diphosphate, role of C221 and E91 | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of wild-type, E91A, E91D and E91Q mutant PDC in Escherichia coli BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E91A | mutant with 30fold reduced specific activity, reduced turnover number and catalytic efficiency, abolished cooperativity, reduced thermal stability, impaired ability to bind the cofactors | Saccharomyces cerevisiae |
E91D | mutant with 5fold reduced specific activity, reduced turnover number and catalytic efficiency, slightly reduced Hill coefficient, reduced thermal stability, impaired ability to bind the cofactors | Saccharomyces cerevisiae |
E91Q | mutant with 4fold reduced specific activity, reduced turnover number and catalytic efficiency, abolished cooperativity, reduced thermal stability, impaired ability to bind the cofactors | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data, pH-dependence of steady-state kinetic parameters | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor, requirement | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence | Saccharomyces cerevisiae |
61320 | - |
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence | Saccharomyces cerevisiae |
61468 | - |
x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type, E91A, E91D and E91Q mutant PDC, expressed in Escherichia coli, mutants are purified as apoenzymes | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2-oxo carboxylate = an aldehyde + CO2 | mechanism | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: pyruvamide | Saccharomyces cerevisiae | ? | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | detailed mechanism | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, x * 61320, mass spectrometry, x * 61468, calculated from the nucleotide sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PDC1 | - |
Saccharomyces cerevisiae |
scpdc1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38.5 | - |
Tm, E91A mutant PDC | Saccharomyces cerevisiae |
42.5 | - |
Tm, E91D mutant PDC | Saccharomyces cerevisiae |
45.5 | - |
Tm, E91Q mutant PDC | Saccharomyces cerevisiae |
59.5 | - |
Tm, wild-type PDC | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
pyruvate | pH 6, 25°C, wild-type PDC | Saccharomyces cerevisiae | |
3.1 | - |
pyruvate | pH 6, 25°C, E91A mutant PDC | Saccharomyces cerevisiae | |
17.5 | - |
pyruvate | pH 6, 25°C, E91D mutant PDC | Saccharomyces cerevisiae | |
20.4 | - |
pyruvate | pH 6, 25°C, E91Q mutant PDC | Saccharomyces cerevisiae | |
73.1 | - |
pyruvate | pH 6, 25°C, wild-type PDC | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement, located at the active site at the interface of the alpha and gamma domains | Saccharomyces cerevisiae |