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Literature summary for 4.1.1.1 extracted from

  • Candy, J.M.; Duggleby, R.G.
    Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme (1998), Biochim. Biophys. Acta, 1385, 323-338.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D440E active, but unlike the wild type enzyme, exhibits a lag phase in product formation which can be reduced by preincubation with 5 mM thiamine diphosphate. Mutant N467D shows decreased affinity for thiamine diphosphate Zymomonas mobilis

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxypyruvate
-
Zymomonas mobilis
phosphate
-
Ipomoea batatas
phosphate
-
Sarcina ventriculi
phosphate
-
Zea mays
phosphate
-
Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Zymomonas mobilis
0.5
-
pyruvate
-
Zea mays
0.5
-
pyruvate
-
Zymomonas mobilis
0.52
-
pyruvate wild type enzyme and mutant enzyme E449D Zymomonas mobilis
0.71
-
pyruvate mutant enzyme H114Q Zymomonas mobilis
0.86
-
pyruvate mutant enzyme W487L Zymomonas mobilis
0.95
-
pyruvate mutant enzyme D440E and mutant enzyme N467D Zymomonas mobilis
0.97
-
pyruvate mutant enzyme F496I Zymomonas mobilis
1.06
-
pyruvate mutant enzyme F496H Zymomonas mobilis
1.33
-
pyruvate mutant enzyme V111A Zymomonas mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharomyces cerevisiae
Mg2+
-
Zea mays
Mg2+
-
Zymomonas mobilis
Mg2+
-
Ipomoea batatas
Mg2+
-
Saccharomyces uvarum
additional information
-
Zymomonas mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pisum sativum the enzyme catalyzes the penultimate step in alcohol fermentation ?
-
?
additional information Zymomonas mobilis the enzyme catalyzes the penultimate step in alcohol fermentation ?
-
?

Organism

Organism UniProt Comment Textmining
Acetobacter sp.
-
-
-
Aspergillus sp.
-
-
-
Canavalia ensiformis
-
-
-
Citrus sp.
-
-
-
Clostridium botulinum
-
-
-
Erwinia amylovora
-
-
-
Hanseniaspora uvarum
-
-
-
Ipomoea batatas
-
-
-
Kluyveromyces sp.
-
-
-
Neurospora crassa
-
-
-
Pastinaca sativa
-
-
-
Pisum sativum
-
cv. Miko
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces pastorianus
-
-
-
Saccharomyces uvarum
-
-
-
Sarcina ventriculi
-
-
-
Schizosaccharomyces pombe
-
-
-
Zea mays
-
-
-
Zymomonas mobilis
-
wild type and mutant enzymes E50D, E50Q, V111A, H113Q, H114Q, D440N, D440T, D440G, D440E, E449D, N467Q, N467D, W487L, F496I, F496H
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Oxobutanoate
-
Zymomonas mobilis ?
-
?
2-Oxopentanoate
-
Zymomonas mobilis ?
-
?
3-Fluoropyruvate decarboxylation is followed by release of F- Saccharomyces cerevisiae acetate + F- + CO2
-
?
3-Fluoropyruvate decarboxylation is followed by release of F- Zymomonas mobilis acetate + F- + CO2
-
?
3-Hydroxypyruvate
-
Zymomonas mobilis Glycolaldehyde + CO2
-
?
additional information the enzyme catalyzes the penultimate step in alcohol fermentation Pisum sativum ?
-
?
additional information the enzyme catalyzes the penultimate step in alcohol fermentation Zymomonas mobilis ?
-
?
pyruvate
-
Saccharomyces pastorianus acetaldehyde + CO2
-
?
pyruvate
-
Saccharomyces cerevisiae acetaldehyde + CO2
-
?
pyruvate
-
Neurospora crassa acetaldehyde + CO2
-
?
pyruvate
-
Pisum sativum acetaldehyde + CO2
-
?
pyruvate
-
Zea mays acetaldehyde + CO2
-
?
pyruvate
-
Schizosaccharomyces pombe acetaldehyde + CO2
-
?
pyruvate
-
Zymomonas mobilis acetaldehyde + CO2
-
?
pyruvate
-
Citrus sp. acetaldehyde + CO2
-
?
pyruvate
-
Acetobacter sp. acetaldehyde + CO2
-
?
pyruvate
-
Aspergillus sp. acetaldehyde + CO2
-
?
pyruvate
-
Canavalia ensiformis acetaldehyde + CO2
-
?
pyruvate
-
Clostridium botulinum acetaldehyde + CO2
-
?
pyruvate
-
Ipomoea batatas acetaldehyde + CO2
-
?
pyruvate
-
Saccharomyces uvarum acetaldehyde + CO2
-
?
pyruvate
-
Pastinaca sativa acetaldehyde + CO2
-
?
pyruvate
-
Kluyveromyces sp. acetaldehyde + CO2
-
?
pyruvate
-
Hanseniaspora uvarum acetaldehyde + CO2
-
?
pyruvate
-
Sarcina ventriculi acetaldehyde + CO2
-
?
pyruvate
-
Erwinia amylovora acetaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
More
-
Saccharomyces pastorianus
More
-
Saccharomyces cerevisiae
More
-
Neurospora crassa
More
-
Zea mays
More
-
Sarcina ventriculi
More phosphate stabilizes the tetramer by shifting the dimer-tetramer equilibrium to higher pH values, without altering the conformation of the tetramer Zymomonas mobilis