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Literature summary for 3.9.1.1 extracted from
- Some properties of beef spleen phosphoamidase (1957), J. Biol. Chem., 229, 111-119.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl compounds | i.e. beta-mercaptoethanol | Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 40% inhibition | Bos taurus | |
| Fe3+ | 30% inhibition | Bos taurus | |
| fluoride | - |
Bos taurus | |
| Iodosobenzene | - |
Bos taurus | |
| p-chloromercuribenzoate | - |
Bos taurus | |
| phosphate | concentration higher than 1 mM | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine phosphate + H2O | Bos taurus | - |
creatine + o-phosphate | - |
? | |
| phosphoramidate + H2O | Bos taurus | cleavage of the N-P bond | NH4+ + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 50fold | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N-phosphocreatine + H2O = creatine + phosphate | also acts on N-phospho-arginine and other phosphoamides | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 38 enzyme units per mg protein with substrate o-carboxyphenylphosphate, 1 enzyme unit defined as amount of enzyme causing absorbance change per 5 min, measurement of salicylic acid | Bos taurus |
| 2.2 | - |
substrate phosvitin, measurement of phosphate | Bos taurus |
| 4.8 | - |
substrate phosphoramidate, measurement of NH4+ | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, lyophilized, 1 month | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-phosphoryl-DL-tryptophan methyl ester + H2O | - |
Bos taurus | DL-tryptophan methyl ester + phosphate | - |
? | |
| creatine phosphate + H2O | - |
Bos taurus | creatine + phosphate | - |
? | |
| creatine phosphate + H2O | - |
Bos taurus | creatine + o-phosphate | - |
? | |
| N-(p-chlorophenyl)phosphoramidate + H2O | - |
Bos taurus | 4-chlorophenylamine + phosphate | - |
? | |
| N-(p-tolyl)phosphoramidate + H2O | - |
Bos taurus | 4-aminophenol + phosphate | - |
? | |
| N-phosphoglycine + H2O | - |
Bos taurus | glycine + phosphate | - |
? | |
| o-carboxyphenylphosphate + H2O | - |
Bos taurus | salicylic acid + phosphate + CO2 | - |
? | |
| O-phospho-L-tyrosine + H2O | - |
Bos taurus | L-tyrosine + phosphate | - |
? | |
| p-nitrophenylphosphate + H2O | - |
Bos taurus | p-nitrophenol + phosphate | - |
? | |
| phosphoramidate + H2O | cleavage of the N-P bond | Bos taurus | NH4+ + phosphate | - |
? | |
| phosvitin + H2O | - |
Bos taurus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
heating for 5 min does not destroy the enzyme activity or precipitate the enzyme | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
about | Bos taurus |
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